超高效液相色谱-串联质谱法检测植物源加工食品中农药残留

Determination of pesticide residues in processed foods of plant origin by ultra performance liquid chromatography tandem mass spectrometry

中文关键词:  植物源加工食品  农药残留  超高效液相色谱-串联质谱法

英文关键词:processed food of plant origin  pesticide residues  ultra performance liquid chromatography tandem mass spectrometry

中文摘要:

      目的 建立超高效液相色谱-串联质谱法检测植物源加工食品中35种农药残留的方法。方法 样品经乙腈提取, QuEChERS方法净化, 用Shim-pack GIST C18色谱柱分离, 以乙腈和0.1％甲酸水溶液为流动相进行梯度洗脱, 电喷雾正离子/负离子模式电离, 多反应监测扫描模式检测, 外标法定量。结果 35种化合物在0.002~0.2 μg/mL范围内均呈现良好的线性关系, 相关系数(r)均大于0.990。检出限为0.04~3.43 μg/kg, 苹果泥样品中平均加标回收率为62%~97%, 相对标准偏差为2.4%～9.1%。结论 本方法重现性好, 回收率高, 可实现植物源加工食品中35种农药残留的同时定性、定量分析。

英文摘要:

      Objective To establish a method for the determination of 35 pesticide residues in processed food of plant origin by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods The samples were extracted with acetonitrile, purified by QuEChERS method, and separated with Shim-pack GIST C18 chromatographic column for gradient elution using acetonitrile and 0.1% formic acid aqueous solution as mobile phase, ionized by electrospray positive/negative ion mode, detected by multiple reaction monitoring scanning mode and quantified by external standard method. Results A total of 35 kinds of compounds had good linear relationships in the range of 0.002?0.2 μg/mL, and the correlation coefficients were larger than 0.990. The limits of detection were 0.04?3.43 μg/kg. The average standard recoveries of apple puree were 62%?97% and the relative standard deviations were 2.4%?9.1%. Conclusion This method has good reproducibility and high recovery rates. It can realize the simultaneous qualitative and quantitative analysis of 35 pesticide residues in processed food of plant origin