微生物法测定婴幼儿乳粉中维生素B12含量的不确定度评定

Uncertainty evaluation for determination of vitamin B12 in infant milk powder by microbiological method

中文关键词:  微生物法  婴幼儿乳粉  维生素B12  不确定度  莱士曼氏乳酸杆菌

英文关键词:microbiological method  infant milk powder  vitamin B12  uncertainty  Lactobacillus leichmannii

中文摘要:

      目的 建立微生物法测定婴幼儿乳粉中维生素B12含量的不确定评定方法。方法 依据JJF 1059.1—2012《测量不确定度评定与表示》及GB 5413.14—2010《食品安全国家标准婴幼儿食品和乳品中维生素B12的测定》分析微生物法测定婴幼儿乳粉中维生素B12含量过程中产生的各测量不确定度分量, 计算测量结果的合成标准不确定度和扩展不确定度。结果 当样品中维生素B12含量为3.97 μg/100 g时, 其扩展不确定度为0.53 μg/100 g(k=2)。结论 标准溶液配制和标准曲线拟合计算待测溶液含量产生的不确定度对检测结果影响较大。

英文摘要:

      Objective To establish an uncertain assessment method for the determination of vitamin B12 in infant milk powder by microbiological method. Methods According to JJF 1059.1—2012 Evaluation and representation of measurement uncertainty and GB 5413.14—2010 National food safety standard for the determination of vitamin B12in infant food and dairy products, the components of measurement uncertainty generated during the determination of vitamin B12 in infant milk powder by microbial method were analyzed, and the synthetic standard uncertainty and extended uncertainty of the measured results were calculated. Results When the vitamin B12 content of the sample was 3.97 g/100 g, the expansion uncertainty was 0.53 g/100 g (k=2). Conclusion Theuncertaintiescaused by the preparation of standard solution and the calculation of standard curve fitting havea great influence on the test results.