饮用天然矿泉水中疑似粪链球菌的鉴定与分析

Identification and analysis of suspected Enterococcus faecalis in drinking natural mineral water

中文关键词:  饮用天然矿泉水  粪链球菌  粘质沙雷菌  VITEK 2 COMPACT全自动微生物鉴定系统

英文关键词:drinking natural mineral water  Enterococcus faecalis  Serratia marcescens  VITEK 2 COMPACT automatic microbial identification system

中文摘要:

      目的 检测某品牌饮用天然矿泉水样品中是否含有粪链球菌, 并对出现的疑似菌落进行分析。方法 按照GB 8538.56-2016《食品安全国家标准 饮用天然矿泉水检验方法》中粪链球菌的检测方法对样品进行检测, 对疑似菌落进行确证性实验, 并通过VITEK 2 COMPACT全自动微生物鉴定系统对疑似菌进行鉴定。 结果 滤膜上出现典型红色菌落, 经确证性实验确认为非粪链球菌; 经VITEK 2 COMPACT鉴定, 该菌为粘质沙雷氏菌。结论 样品中的疑似菌落非粪链球菌而是粘质沙雷菌。

英文摘要:

      Objective To detect the presence of Enterococcus faecalis in a natural mineral water sample and analyze the suspected bacterial colonies. Methods The samples were tested in accordance with the test method specified in GB 8538-2016 National food safety standard-Inspection of natural mineral water for drinking, and the suspected bacterial colonies were confirmed by VITEK 2 COMPACT automatic microbial identification system. Results The typical red colony appeared on the membrane, which was confirmed as non-streptococcus faecalis by confirmative test. VITEK 2 COMPACT identified the bacteria as Serratia marcescens. Conclusion The suspected colony of Enterococcus faecalis in the samples is not Enterococcus faecalis but Serratia marcescens.