苹果中阿维菌素的残留分析与消解动态研究

Study on abamectin residue analysis and digestion dynamics in apples

中文关键词:  阿维菌素  苹果  消解动态  残留  高效液相色谱法

英文关键词:avermectin  apples  digestion dynamics  residual  high performance liquid chromatography

中文摘要:

      目的 探究12.5%阿维·哒螨灵(0.25%阿维菌素+12.25%哒螨灵)可湿性粉剂成分之一阿维菌素在苹果和土壤中的残留及消解动态。方法 分别在北京、安徽、陕西3地种植的苹果和土壤中进行阿维菌素最终残留实验及消解动态实验。样品经乙腈提取、盐析、浓缩处理后, 采用高效液相色谱-荧光法检测阿维菌素的残留量。结果 该方法检出限为0.02 ng, 定量限为0.001 mg/kg, 在苹果和土壤中的添加回收率分别为88.7%~94.2%和90.5%~96.4%, 相对标准偏差分别为3.56%~6.01%和3.43%~7.25%。阿维菌素在苹果和土壤中的半衰期分别为2.6~4.4 d和3.4~6.2 d。在推荐使用高剂量和推荐使用高剂量1.5倍的情况下, 施药后7、14、21、28 d阿维菌素在苹果上的残留量均小于MRL值0.02 mg/kg。结论 12.5%阿维·哒螨灵可湿性粉剂其之一成分阿维菌素在苹果上的安全间隔期可建议定为7 d。

英文摘要:

      Objective To investigate the residue and digestion dynamics of abamectin, one of the wettable powder components of 12.5% abamectin pyridaben (0.25% abamectin+12.25% pyridaben) in apples and soil. Methods The final residue and digestion dynamic experiments of abamectin were carried out in apples and soil planted in Beijing, Anhui and Shaanxi. The samples were extracted with acetonitrile, salted out and concentrated, the residual abamectin was determined by high performance liquid chromatography-fluorescence method. Results The limit of detection and limit of quantitative of this method were 0.02 ng and 0.001 mg/kg, respectively. The recoveries of addition in apples and soil were 88.7%?94.2% and 90.5%?96.4% respectively, and the relative standard deviations were 3.56%?6.01% and 3.43%?7.25% respectively. The half-lives of abamectin in apple and soil were 2.6?4.4 d and 3.4?6.2 d, respectively.At the recommended high dose and 1.5 times of the recommended high dose, the residual abamectin in apples at 7, 14, 21 and 28 days after application were less than the MRL value of 0.02 mg/kg. Conclusion The safety interval of abamectin, one of its ingredients, on apples can be recommended as 7 days.