表面增强拉曼光谱法快速测定液态乳中三聚氰胺方法的研究和评价

Research and evaluation of surface enhanced Raman spectroscopy in the rapid determination of melamine in liquid milk

中文关键词:  表面增强拉曼光谱  三聚氰胺  牛奶

英文关键词:surface enhanced Raman spectroscopy  melamine  milk

中文摘要:

      目的 建立便携式拉曼光谱法定性检测液态乳中三聚氰胺的快速检验方法。方法 选择市场上有代表性的拉曼光谱仪, 确定检测方法。探究增强试剂、常见的添加剂及原料对检测结果的影响以及三聚氰胺类似物对检测结果的影响, 确定方法的适用范围并被验证。对指定的3家验证机构进行方法验证。结果 5家企业的快速检测产品中有3家在验证中完全满足方法的性能指标, 1家企业的产品的2次验证实验中有1次不符合方法的性能指标, 1家企业的产品3次验证实验中有2次不符合方法的性能指标。3次不符合方法的性能指标的验证实验仅在灵敏度上略差。结论 本方法各项性能指标符合要求, 可以应用于液态乳中三聚氰胺的快速检测。

英文摘要:

      Objective To establish a fast method for the qualitative determination of melamine in liquid milk by portable Raman spectrometric. Methods The representative Raman spectrometer on the market was selected, and the detection method was determined. The influence of reinforcing reagent, common additives and raw materials on the test results and the influence of melamine analogues on the test results were explored, and the applicable scope of the method were determined and verified. The method validation was performed for the three specified verification agencies. Results Among the rapidly tested products, three out of five enterprises fully met the performance indexes of the method in the verification. One enterprise's products failed to meet the performance index of the method in two verification experiments. Two enterprise's products failed to meet the performance index of the method in three verification experiments. The three verification experiments of performance indexes that do not conform to the method were only slightly less sensitive. Conclusion The method can be applied to the rapid detection of melamine in liquid milk.