殷秋妙,赵亚荣,王威利,季天荣,黄晓梅,丁晨红.奶粉中沙门氏菌和阪崎肠杆菌的测定能力验证结果分析[J].食品安全质量检测学报,2020,11(24):9336-9342 |
奶粉中沙门氏菌和阪崎肠杆菌的测定能力验证结果分析 |
Proficiency testing results analysis of determination of Salmonella and Enterobacter sakazakii in powder milk |
投稿时间:2020-08-06 修订日期:2020-12-07 |
DOI: |
中文关键词: 沙门氏菌 阪崎肠杆菌 乳粉 |
英文关键词:Salmonella Enterobacter sakazakii milk powder |
基金项目:农业农村部重点实验室建设服务与管理提升项目(粤财农〔2020〕100号) |
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Author | Institution |
YIN Qiu-Miao | Public Monitoring Center for Agro-product, Guangdong Academy of Agricultural Science; Laboratory of Quality and Safety Risk Assessment of Agro-Product (Guangzhou), Ministry of Agriculture |
ZHAO Ya-Rong | Public Monitoring Center for Agro-product, Guangdong Academy of Agricultural Science; Laboratory of Quality and Safety Risk Assessment of Agro-Product (Guangzhou), Ministry of Agriculture |
WANG Wei-Li | Public Monitoring Center for Agro-product, Guangdong Academy of Agricultural Science; Laboratory of Quality and Safety Risk Assessment of Agro-Product (Guangzhou), Ministry of Agriculture |
JI Tian-Rong | Public Monitoring Center for Agro-product, Guangdong Academy of Agricultural Science; Laboratory of Quality and Safety Risk Assessment of Agro-Product (Guangzhou), Ministry of Agriculture |
HUANG Xiao-Mei | Public Monitoring Center for Agro-product, Guangdong Academy of Agricultural Science; Laboratory of Quality and Safety Risk Assessment of Agro-Product (Guangzhou), Ministry of Agriculture |
DING Chen-Hong | Public Monitoring Center for Agro-product, Guangdong Academy of Agricultural Science; Laboratory of Quality and Safety Risk Assessment of Agro-Product (Guangzhou), Ministry of Agriculture |
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中文摘要: |
目的 提高实验室使用传统生化方法检测沙门氏菌和克罗诺杆菌属(阪崎肠杆菌)的能力和水平。 方法 用空白添加法和交叉试验法检查培养基性能后, 以奶粉样品为材料, 用传统生化培养方法进行检测, 同时对可疑菌落进行辅助生化鉴定。结果 W样品检出沙门氏菌, Q样品未检出沙门氏菌; V样品检出阪崎肠杆菌, G样品未检出阪崎肠杆菌, 检测结果满意。结论 本实验室的沙门氏菌和克罗诺杆菌属(阪崎肠杆菌)的传统生化检测技术水平和能力得到了提高, 为同类实验室检测能力的提高提供参考。 |
英文摘要: |
Objective To improve the laboratory's ability and level to detect Salmonella and Kronobacter (Enterobacter sakazakii) using traditional biochemical methods. Methods After checking the performance of the culture medium by the blank addition method and the crossover test method, the milk powder sample was used as the material, and the traditional biochemical culture method was used for detection, and the suspicious colony was assisted by biochemical identification. Results Salmonella was detected in W sample, and not detected in Q sample; Enterobacter sakazakii was detected in V sample, and not detected in sample G. The test results were satisfactory. Conclusion The traditional biochemical detection technology and capabilities of Salmonella and Kronobacter (Enterobacter sakazakii) in this laboratory have been improved, providing a reference for the improvement of the detection capabilities of similar laboratories. |
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