QuEChERS-高效液相色谱法检测不同畜禽产品中5种氟喹诺酮类的药物残留

Determination of 5 fluoroquinolones residues in different livestock and poultry products by QuEChERS-high performance liquid chromatography

中文关键词:  QuEChERS  畜禽产品  氟喹诺酮类药物

英文关键词:QuEChERS  livestock and poultry products  fluoroquinolones

中文摘要:

      目的 建立QuEChERS法检测多种畜禽产品中5种氟喹诺酮类药物残留。方法 采用酸化的乙腈溶液提取猪、牛、羊、鸡和鸭等畜禽的肌肉、皮脂、脂肪、肝和肾组织中氟喹诺酮类残留, 用微型高速分散机在离心管内同时完成均质与提取, 采用增强型脂质去除过滤柱对提取物进行2次洗脱, 用高效液相色谱-可变波长荧光检测器法检测。结果 达氟沙星和恩诺沙星的线性范围为5~500 μg/L, 沙拉沙星的线性范围为2.5~100.0 μg/L, 二氟沙星的线性范围为10~2000 μg/L, 氟甲喹的线性范围为10~3000 μg/L; 检出限为2~ 10 μg/kg, 定量限为5~25 μg/kg。畜禽产品基质中各药物的空白样品加标回收率均为76.09%~93.20%, RSDs为2.98%~6.29%。结论 该方法快速、准确、灵敏, 适合测定猪、牛、羊、鸡和鸭的肌肉、脂肪(皮脂)、肾和肝中沙拉沙星、恩诺沙星、达氟沙星、二氟沙星和氟甲喹药物残留。

英文摘要:

      Objective To establish a method for the determination of 5 fluoroquinolones residues in various livestock and poultry products by QuEChERS method. Methods Fluoroquinolones were extracted from the muscle, sebum, fat, liver and kidney tissues of pigs, cattle, sheep, chickens and ducks by acidified acetonitrile solution. Homogenization and extraction were performed simultaneously in a centrifuge tube with a micro high speed dispersing machine. The extract was eluted twice with an enhanced lipid removal filter column and detected by high performance liquid chromatogram-variable wavelength fluorescence detector. Results The linear ranges of dafloxacin and enfloxacin were 5?500 μg/L, the linear range of sarfloxacin was 2.5?100.0 μg/L, the linear range of difloxacin was 10?2000 μg/L, and the linear range of flumequine was 10?3000 μg/L. The limits of detection were 2-10 μg/kg, and the limits of quantitation were 5?25 μg/kg. The standard recoveries of blank samples and RSDs were 76.09%?93.20% and 2.98%?6.29%, respectively. Conclusion This method is rapid, accurate and sensitive, which is suitable for the determination of sarfloxacin, enfloxacin, daffloxacin, difloxacin and flumequine drug residues in muscle, fat (sebum), kidney and liver of pig, ox, sheep, chicken and duck.