高效液相色谱法同时测定奶昔粉中的安赛蜜和瑞鲍迪苷A

Determination of acesulfame potassium and rebaudioside A in milkshake powder by high performance liquid chromatography

中文关键词:  奶昔粉  安赛蜜  瑞鲍迪苷A  高效液相色谱法

英文关键词:milkshake powder  acesulfame potassium  rebaudioside A  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法(high performance liquid chromatography, HPLC)同时快速检测奶昔粉中安赛蜜和瑞鲍迪苷A含量的分析方法。方法 样品用水溶解稀释, 加入沉淀剂, 离心过滤得样品供试液。采用C18色谱柱, 以硫酸铵溶液:乙腈:甲醇=65:30:5(V:V:V)作为流动相, 检测波长为210 nm, 流速 0.6 mL/min, 进行检测, 外标法定量。结果 本方法在20 min内完成2种目标化合物的分离分析。试样中安赛蜜浓度在0.0040~0.0403 mg/mL的范围内与峰面积的线性关系良好, 相关系数r＞0.999, 在3水平不同浓度安赛蜜标准溶液添加下, 安赛蜜的回收率为98.58%。试样中瑞鲍迪苷A浓度在0.0028~0.0283 mg/mL的范围内与峰面积的线性关系良好, 相关系数r＞0.999。在3水平不同浓度瑞鲍迪苷A标准溶液添加下, 瑞鲍迪苷A的回收率为99.04%。均符合标准GB/T 27404-2008《实验室质量控制规范 食品理化检测》中的要求, 且目标峰分离效果更好, 效率提升49.5%。结论 该方法快速、准确、灵敏, 适合同时测定奶昔粉中安赛蜜和瑞鲍迪苷A含量。

英文摘要:

      Objective To establish a method for simultaneous determination of acesulfame potassium and rebaudioside A in milkshake powder by high performance liquid chromatography. Methods The sample was diluted with water, after the precipitant was added, the sample was centrifuged and filtered to obtain the sample test solution. Ammonium sulfate solution:acetonitrile:methanol=65:30:5 (V:V:V) was used as mobile phase on a C18 column, the detection wavelength was 210 nm and the flow rate was 0.6 mL/min for detection, and the external standard method was used for quantitative analysis. Results Two target compounds were separated and analyzed in 20 min. In the sample, there was a good linear relationship between the concentration of acesulfame potassium and the peak area in the range of 0.0040?0.0403 mg/mL, and the correlation coefficient was greater than 0.999. The recovery rate of acesemide was 98.58% when the standard solution with different concentrations of acesulfame potassium was added at 3 levels. There was a good linear relationship between the concentration of rebaudioside A and the peak area in the range of 0.0028?0.0283 mg/mL, and the correlation coefficient was greater than 0.999. The recovery rate of acesemide was 98.58% when the standard solution with different concentrations of rebaudioside A was added at 3 levels. All of them met the requirements of the standard GB/T 27404?2008 Laboratory quality control standard-Physical and chemical testing of food, the separation effect of target peak was better, and the efficiency is increased by 49.5%. Conclusion The proposed method is fast, accurate and sensitive, which is suitable for the simultaneous determination of acesulfame potassium and rebaudioside A in milkshake powder.