非衍生化液相色谱-串联质谱法测定茶叶中草铵膦残留量

Determination of glufosinate ammonium residue in tea by nonderivative liquid chromatography-tandem mass spectrometry

中文关键词:  茶叶  草铵膦  非衍生化  液相色谱-串联质谱法

英文关键词:glufosinate-ammonium  non derivatization  liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立非衍生化液相色谱-串联质谱法测定茶叶中草铵膦残留的分析方法。方法 茶叶样品经超纯水均质提取, HR-X固相萃取小柱净化, 采用非衍生化液相色谱-串联质谱法直接测定, 电喷雾离子源负离子模式下采用多反应监测模式监测, 同位素内标法定量。结果 在优化的条件下, 草铵膦在5~200 μg/L范围内呈良好的线性关系, 相关系数为0.9998, 方法定量限为0.1 mg/kg。方法平均回收率为84.2%~104.9%, 相对标准偏差低于7.6%。结论 本方法前处理简单、准确性好、灵敏度高, 可用于绿茶、红茶、乌龙茶、普洱茶等茶叶中草铵膦残留量的测定。

英文摘要:

      Objective To establish a method for the determination of glufosinate ammonium residue in tea by nonderivative liquid chromatography-tandem mass spectrometry. Methods Tea samples were homogenized and extracted by ultra-pure water and purified by HR-X solid phase extraction column. The contents were directly detected by nonderivatization liquid chromatography-tandem mass spectrometry, monitored by multi-reaction monitoring method under the negative ion mode of electrospray ion source, and quantified by isotopic internal standard method. Results Under optimized conditions, glufosinate-ammonium showed a good linear relationship in the range of 5?200 μg/L, and the correlation coefficient was 0.9998, and limit of quantification was 0.1 mg/kg. The average recoveries were 84.2%?104.9% and the relative standard deviations were less than 7.6%. Conclusion The proposed method is simple, accurate and sensitive, which is suitable for detecting glufosinate-ammonium residue in green tea, black tea, oolong tea and Pu'er tea.