高效液相色谱法测定红参中12种人参皂苷的含量

Determination of 12 ginsenosides in red ginseng by high performance liquid chromatography

中文关键词:  红参  高效液相色谱法  人参皂苷

英文关键词:red ginseng  high performance liquid chromatography  ginsenoside

中文摘要:

      目的 建立高效液相色谱法(high performance liquid chromatography, HPLC)测定红参中12种人参皂苷含量的方法。方法 样品提取后, 经Thermo Hypersil C18色谱柱(250 mm×4.6 mm, 5 μm)分离, 以乙腈-0.1%磷酸水溶液为流动相进行梯度洗脱, 流速为1.0 mL/min, 柱温40 ℃, 检测波长203 nm, 外标法定量。结果 12种人参皂苷在一定质量浓度范围内线性关系良好(r≥0.9999), 平均回收率为95.9%~99.4%, 相对标准偏差为0.10%~1.30%。29批红参药材均含12种人参皂苷。批次间比较分析, 12种人参皂苷含量差异较大, 说明红参质量参差不等。结论 该方法准确度高, 重复性好, 可为红参的质量评价提供参考。

英文摘要:

      Objective To establish a method for determination of 12 ginsenosides in red ginseng by high performance liquid chromatography. Methods After extraction, the samples were separated by Thermo Hypersil C18 column (250 mm×4.6 mm, 5 μm) using acetonitrile-0.1% phosphoric acid solution as the mobile phase for gradient elution. The flow rate was 1.0 mL/min, the column temperature was 40 ℃, and the detection wavelength was 203 nm. The samples were quantified by external standard method. Results The 12 ginsenosides had good linear relationshipes within the certain mass concentration range (r≥0.9999), the average recovery rates were 95.9%?99.4%, and the relative standard deviations were 0.10%-1.30%. Totally 29 batches of red ginseng medicinal materials all contained 12 kinds of ginsenosides. Comparative analysis between batches showed that the content of 12 ginsenosides was quite different, indicating that the quality of red ginseng varied. Conclusion This method has high accuracy and good repeatability, which can provide reference for the quality evaluation of red ginseng.