6种市售酶抑制-比色法试剂盒测定有机磷农药的比较研究

Comparative study on the determination of organo-phosphorus pesticides with 6 kinds of commercially available enzyme inhibition colorimetric kits

中文关键词:  试剂盒  农药残留  抑制率  敏感性  半抑制浓度  阳性样品

英文关键词:test kit  pesticide residue  inhibition rate  sensibility  half-inhibitory concentration  positive samples

中文摘要:

      目的 探讨6种市售农残速测试剂盒对倍硫磷氧乐果等5种有机磷类农药的敏感性。方法 使用6种市售酶抑制-比色法农残速测试剂盒分别在5个浓度水平下测定了倍硫磷等5种农药的抑制率, 绘制抑制率对农药浓度的折线图, 同时用抑制率和农药浓度的常用对数进行线性拟合, 分别计算出相关系数、半抑制浓度(half-inhibitory concentration, IC50)和IC10, 根据IC10比较各种试剂盒对每种农药的敏感性。结果 6种试剂盒对倍硫磷均不敏感, 6种试剂盒对其他4种农药的敏感性也各不相同, 从所测定的5种农药来看, D试剂盒最优。结论 建议国家标准和行业标准将判定阳性检出样品的标准降低。

英文摘要:

      Objective To investigate the sensitivity of 6 kinds of pesticide residues test kits to 5 kinds of organophosphorus pesticides, such as fenthion. Methods Total of 6 commercially available enzyme inhibition colorimetric assay kits were used to determine the inhibition rates of 5 pesticides such as fenthion at 5 concentration levels, a line graph of inhibition rate versus pesticide concentration was drawn, and the common logarithm of inhibition rate and pesticide concentration was used for linear fitting. The correlation coefficient, the half-inhibitory concentration (IC50) and IC10 were calculated. According to IC10, the sensitivity of various kits to each pesticide was compared. Results The 6 kits were not sensitive to fenthion, and the sensitivity of the 6 kits to other 4 pesticides was also different. According to the 5pesticides determined, D kit was the best. Conclusion It is suggested that the national standard and industrial standard should reduce the standard of positive detection samples.