高效液相色谱法测定保健食品中的羟基酪醇

Determination of hydroxytyrosol in health food by high performance liquid chromatography

中文关键词:  保健食品  羟基酪醇  高效液相色谱法

英文关键词:health food  hydroxytyrosol  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法测定保健食品中羟基酪醇的方法。方法 样品用二甲基亚砜进行超声提取后, 采用Waters Spherisorb ODS2色谱柱(250 mm×4.6 mm, 5 μm)进行分离, 以0.12%甲酸水溶液?0.12%甲酸甲醇溶液为流动相等度洗脱, 柱温为30 ℃, 流速1 mL/min, 紫外检测器进行检测, 外标法定量。结果 羟基酪醇在12.45～498.0 μg/mL范围内线性关系良好, 相关系数大于0.999; 检出限为0.03 g/100 g; 定量限为 0.12 g/100 g; 平均回收率为99.5%~100.6%, 相对标准偏差值为0.55%~1.13%。结论 该方法简便、快捷、高效、准确, 适用于保健食品中羟基酪醇的测定。

英文摘要:

      Objective To establish a method for the determination of hydroxytyrosol in health food by high performance liquid chromatography. Methods After ultrasonic extraction with dimethyl sulfoxide, the samples were separated with Waters Spherisorb ODS2 column (250 mm×4.6 mm, 5 μm) by using 0.12% formic acid aqueous solution-0.12% formic acid methanol solution as mobile phase for isocratic elution at the flow of 1 mL/min. The temperature of column was 30 ℃. The samples were detected by UV detector and quantified by external standard method. Results Hydroxytyrosol had a good linear relationship in the range of 12.45?498.0 μg/mL, and the correlation coefficient was greater than 0.999. The limit of detection was 0.03 g/100 g. The limit of quantitation was 0.12 g/100 g. The average recoveries were 99.5%?100.6%, and the relative standard deviations were 0.55%?1.13%. Conclusion This method is simple, rapid, efficient and accurate, which can be applied to detect hydroxytyrosol in health food.