高效液相色谱法测定大豆磷脂类保健食品中的磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰肌醇

Determination of phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol in soybean phospholipid health food by high performance liquid chromatography

中文关键词:  大豆磷脂  保健食品  磷脂酰胆碱  磷脂酰乙醇胺  磷脂酰肌醇  高效液相色谱法

英文关键词:soybean phospholipids  health food  phosphatidylcholine  phosphatidylethanolamine  phosp hated ylinositol  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱检测法测定大豆磷脂类保健食品中磷脂酰胆碱(phosphatidylcholines, PC)、磷脂酰乙醇胺(phosphatidylethanolamine, PE)、磷脂酰肌醇(phosphatidylinositol, PI)含量的方法。方法 大豆磷脂类保健食品试样经正己烷: 异丙醇(1:1, V:V)提取, 经氨基柱(250 mm×4.6 mm, 5 μm)分离, 以流动相为无水乙醇-乙腈-水(50:40:10, V:V:V)为流动相等度洗脱, 流速为0.4 mL/min, 检测波长为205 nm, 外标法定量。结果 磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰肌醇在一定浓度范围内线性关系良好, 相关系数大于0.999; 检出限分别为1.3、5.0、1.0 mg/g, 加标回收率分别为95.5%、99.5%、94.9%, 精密度均为0.3%。结论 本方法准确度高、重复性好, 适用于保健食品中磷酯类物质含量的测定。

英文摘要:

      Objective To establish a method for determination of phosphatidylcholines (PC), phos phatidyl etha nolamine (PE) and phosphatidylinositol (PI) in soybean phospholipid health food by high performance liquid chromatography. Methods The health food samples of soybean phospholipids were extracted by n-hexane: isopropanol(1:1, V:V) and separated with an amino column (250 mm×4.6 mm, 5 μm) using anhydrous ethan ol-acet onitrile-water (50:40:10, V:V:V) as mobile phase for gradient elution at the flow rate of 0.4 mL/min, the detection wavelength was 205 nm, and the contents were quantified by external standard method. Results Phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol had a good linear relationship within a certain concentration range, and the correlation coefficients were greater than 0.999. The limits of detection were 1.3, 5.0, 1.0 mg/g, the recovery rates of addition were 95.5%, 99.5%, 94.9%, respectively, and the precision was 0.3%. Conclusion This method has high accuracy and good repeatability, and is suitable for the determination of phosphorus esters in health food.