微生物法测定全营养配方食品中生物素的不确定度评定

Uncertainty evaluation for the determination of biotin in nutritionally complete food by microbial method

中文关键词:  全营养配方食品  微生物法  生物素  不确定度  非线性曲线拟合

英文关键词:nutritionally complete food  microbial method  biotin  uncertainty  nonlinear curve fitting

中文摘要:

      目的 评定微生物法测定全营养配方食品中生物素的不确定度。方法 依据GB 5009.259-2016 《食品安全国家标准 食品中生物素的测定》和JJF 1059.1-2012 《测量不确定度评定与表示》以及相关统计学方法, 对影响全营养配方食品中生物素含量结果的不确定度分量进行探究和评定。结果 生物素含量的扩展不确定度为1.84 μg/100 g, k=2。通过研究发现测量重复性和标准物质称量及标准溶液配制过程是影响检测结果的主要因素, 试样系列管制备过程、标准曲线拟合、紫外分光光度计的偏差和样液制备过程为次要因素。 结论 该方法灵敏度高, 准确度好, 对全营养配方食品中生物素测定结果的不确定度作出较好评估。

英文摘要:

      Objective To evaluate the uncertainty for determination of biotin in nutritionally complete food by microbiological method. Methods According to GB 5009.259-2016 National food safety standard-Determination of biotin in foods and JJF 1059.1?2012 Evaluation and representation of measurement uncertainty and relevant statistical methods, the uncertainty components affecting biotin content results in nutritionally complete food were explored and evaluated. Results The extended uncertainty of biotin content was 1.84 μg/100 g, k=2. It was found that the repeatability of measurement, the weight of standard substance and the preparation process of standard solution were the main factors affecting the test results. Sample series control preparation process, standard curve fitting, deviation of UV spectrophotometer and sample preparation process were secondary factors. Conclusion This method had high sensitivity and good accuracy, and can be used to evaluate the uncertainty of biotin determination results in whole-nutrition formula food.