元素分析-同位素质谱法鉴定麦卢卡蜂蜜中碳-4植物糖掺假

Identification of Manuka honey C-4 sugars adulteration by elementary analysis-isotope ratio mass spectrometry

中文关键词:  元素分析-同位素质谱法  麦卢卡蜂蜜  掺假  碳-4植物糖

英文关键词:element analyzer stable isotope proportional mass spectrometry  Manuka honey  adulteration  C-4 sugar

中文摘要:

      目的 建立元素分析-同位素质谱法(element analyzer stable isotope proportional mass spectrometry, EA-IRMS)鉴定麦卢卡蜂蜜中碳-4植物糖掺假的新方法。方法 样品经前处理后, 利用元素分析-同位素质谱仪测定蜂蜜的碳同位素比值δ13CH、蜂蜜中总蛋白质的碳同位素比值δ13CP、蜂蜜中花粉的碳同位素比值δ13CF。用麦卢卡蜂蜜中δ13C值更为稳定的δ13CF值为标准, δ13CH值与其进行比较, 参照GB/T 18932.1-2002《蜂蜜中碳-4植物糖含量测定方法 稳定碳同位素比率法》计算方式, 来建立新的计算麦卢卡蜂蜜中碳-4植物糖含量X(%)的计算方式。结果 对纯正麦卢卡蜂蜜提出: 0

英文摘要:

      Objective To establish a method for the determination of C-4 sugar adulteration in Manuka honey by elementary analysis-isotope ratio mass spectrometry. Methods After pretreatment, the carbon isotope value (δ13CH) of honey, total protein (δ13CP) in honey and (δ13CF) pollen in honey were determined by elementary analysis-isotope ratio mass spectrometry. The δ13CF value with more stable δ13C value in Manuka honey was used as the standard, and the δ13CH value was compared with it, and the calculation method of GB/T 18932.1-2002 Determination of C-4 plant sugar content in honey-Stable carbon isotope ratio method was used to establish a new calculation method of C-4 plant sugar content X(%) in Manuka honey. Results The authentic Manuka honey were proposed: 0