响应面法优化核桃分心木中多糖的提取工艺

Optimization of extraction process of polysaccharide from Diaphragma juglandis Fructus by response surface method

中文关键词:  核桃分心木  多糖  超声提取  响应面法

英文关键词:Diaphragma juglandis Fructus  polysaccharides  extraction parultrasons  response surface

中文摘要:

      目的 优化核桃分心木中多糖的提取工艺。方法 采用超声提取法对核桃分心木中多糖进行提取, 以多糖含量为考察指标, 在单因素实验的基础上, 研究超声提取温度、超声提取时间、料液比对核桃分心木多糖含量的影响, 并采用响应面法优化核桃分心木多糖含量的提取工艺。结果 核桃分心木中提取多糖的最佳工艺参数为: 超声提取温度为60 ℃、超声提取时间为40 min、料液比为1:15(m/V)。采用此条件此方法提取核桃分心木中多糖, 含量为17.42%, 相对标准偏差为0.273%。结论 采用响应面优化法得到的提取条件参数准确可靠, 具有实际应用价值。

英文摘要:

      Objective To optimize the extraction process of polysaccharide from Diaphragmajuglandis Fructus. Methods Polysaccharide were extracted from Diaphragma juglandis Fructus by ultrasonic extraction. Taking the polysaccharide content as an index, the effects of ultrasonic extraction temperature, ultrasonic extraction time and feed-liquid ratio on the extraction process of polysaccharide from Diaphragma juglandis Fructus were studied on the basis of single-factor experiment, and the extraction process of polysaccharide from Diaphragma juglandis Fructus was optimized by response surface method. Results The optimum extraction parameters of polysaccharide from Diaphragma juglandis Fructus were as follow: ultrasonic extraction temperature was 60 ℃, ultrasonic extraction time was 40 min, the soild liquid ratio was 1:15(m/V). Under these condition, the polysaccharide content increased to 17.42%, and relative standard deviation was 0.27%. Conclusions The parameters obtained by response surface optimization method are accurate and reliable, and have practical application value.