高效液相色谱法测定熟肉制品中8种合成着色剂

Simultaneous determination of 8 synthetic colorants in cooked meat by high performance liquid chromatography

中文关键词:  合成着色剂  高效液相色谱法  熟肉制品

英文关键词:synthetic colorants  high performance liquid chromatography  cooked meat

中文摘要:

      目的 建立高效液相色谱法同时快速测定熟肉制品中8种合成着色剂的检测方法。方法 称取粉碎后的样品于离心管中, 加入碱性乙醇溶液提取合成着色剂, 用硫酸溶液和钨酸钠溶液去除蛋白, 滤液经浓缩后定容。样液过滤后经Agilent C18( 5 μm, 250 mm×4.6 mm)色谱柱分离, , 流动相为0.02 mol/L NH4Ac(pH 6.86)和甲醇, 流速为1.0 mL/min, 柱温40 ℃。采用二极管阵列检测器在254 nm波长处进行检测, 外标法定量。 结果 该方法在0.5~10.0 mg/kg线性范围内(靛蓝为1.0~20.0 mg/kg)线性良好, 相关系数均大于0.990, 检出限为0.1～1.0 μg/kg, 平均回收率为80.8%～104.7%, 相对标准偏差为4.29%～4.87%。结论 该方法操作简单、灵敏度高、重复性好, 满足同时检测熟肉制品中8种合成着色剂的要求。

英文摘要:

      Objective To establish a method for simultaneous determination of 8 kinds of synthetic colorants in cooked meat by high performance liquid chromatography (HPLC). Methods The sample was weighed after grinding and put into a centrifuge tube, the basic ethanol solution was added to extract and synthesize the colorant. The protein was removed by sulfuric acid solution and sodium tungstate solution. The filtrate was concentrated and constant volume. The sample solution was filtered and separated by Agilent C18 column (5 μm, 250 mm×4.6 mm) with 0.02 mol/L NH4Ac(pH 6.86) and methyl alcohol as mobile phase at 1.0 mL/min flow rate at 40 ℃．The sample solution was detected by diode array detector at 254 nm wavelength and quantified by external standard method. Results The method had a good linearity in the range of 0.5–10.0 mg/kg(indigo was 1.0–20.0 mg/kg) with correlation coefficients (r2) more than 0.990. The detection limits ranged from 0.1–1.0 μg/kg. The average recoveries were in the range of 80.8%–104.7% with relative standard deviations(RSDs) 4.29%–4.87%. Conclusion The method is simple, sensitive and reproducible, which can meet the requirements of simultaneous detection of 8 synthetic colorants in cooked meat products.