高效液相色谱法测定保健品益生菌粉中三氯蔗糖的不确定度评估

Uncertainty evaluation for the determination of sucralose in probiotic powder of health food by high performance liquid chromatography

中文关键词:  高效液相色谱法  保健品  益生菌粉  三氯蔗糖  不确定度

英文关键词:high performance liquid chromatography  health food  probiotic powder  sucralose  uncertainty

中文摘要:

      目的 评定高效液相色谱法(high performance liquid chromatography, HPLC)测定保健品益生菌粉中三氯蔗糖含量的不确定度。方法 依据CNAS-GL 006:2019 《化学分析中不确定度的评估指南》和JJF 1059.1-2012《测量不确定度评定与表示》, 采用HPLC测定保健品益生菌粉中三氯蔗糖含量, 建立不确定度评定的数学模型,通过对测量重复性、标准品溶液配制、样品溶液的配制、高效液相色谱仪以及标准曲线拟合测量偏差这5个方面引入的不确定度进行分析, 并确定各个不确定度分量, 得到扩展不确定度。结果 按置信区间为95%, 保健品益生菌粉中三氯蔗糖含量为(0.78±0.0384) g/kg(k=2)。结论 高效液相色谱法因素对测量不确定度影响显著, 由检验方法重复测量和标准品溶液配制体现。

英文摘要:

      Objective To evaluate the uncertainty for determination of sucralose in probiotic power of health food by high performance liquid chromatography (HPLC). Methods According to CNAS-GL 006:2019 Guidance on evaluating the uncertainty in chemical analysis and JJF 1059.1-2012 Evaluation and expression of measurement uncertainty, the sucralose in probiotic power of health food was measured by HPLC, and the mathematical model of uncertainty was established. The uncertainty was analyzed from 5 aspects, including the repeatability of determination, the preparation of standard solution, the preparation of sample solution, high performance liquid chromatography and the measurement deviation of standard curve fitting. The extended uncertainty was obtained by determining each uncertainty component. Results The confidence interval was 95%, and the content of sucralose in probiotic power was (0.78±0.0384) g/kg, (k=2). Conclusion The influence of the factors of high performance liquid chromatography on the measurement uncertainty is obvious, which is reflected by repeated measurement of test method and preparation of standard-solution.