超高效液相色谱-串联质谱同位素内标法测定水产品中氟苯尼考及其代谢物残留量的不确定度评定

Uncertainty evaluation of determination of florfenicol and its metabolite in aquatic products by ultra performance liquid chromatography-tandem mass spectrometry with isotope-labelled internal standards

中文关键词:  超高效液相色谱-串联质谱法  同位素内标法  水产品  氟苯尼考  氟苯尼考胺  不确定度评定

英文关键词:ultra performance liquid chromatography-tandem mass spectrometry  isotope-labelled internal standards  aquatic products  florfenicol  florfenicol amine  uncertainty evaluation

中文摘要:

      目的 采用超高效液相色谱-串联质谱内标法对水产品中氟苯尼考及其代谢产物氟苯尼考胺的残留量进行测量不确定度评定。方法 根据JJF 1059.1-2012《测量不确定度的评定与表示》等相关理论, 通过建立数学模型, 分析各不确定度来源, 并进行量化, 得到综合标准不确定度。结果 当氟苯尼考含量为 11.0 μg/kg时, 其扩展不确定度为1.09 μg/kg (k＝2); 当氟苯尼考胺含量为7.25 μg/kg时, 其扩展不确定度为0.94 μg/kg (k＝2)。结论 本方法的不确定度主要来自标准溶液配制和曲线拟合。

英文摘要:

      Objective To evaluate the uncertainty for determination of florfenicol and its metabolite florfenicol amine in aquatic products by ultra performance liquid chromatography-tandem mass spectrometry with isotope-labelled internal standards. Methods According to the theory of JJF 1059.1-2012 Evaluation and expression of uncertainty in measurement and other related theories, a mathematical model was established, then the sources of uncertainty were analyzed and quantified, the uncertainty components was calculated to obtain the synthetic standard uncertainty. Results When the florfenicol residue in aquatic products was 11.0 μg/kg, the expanded uncertainty was 1.09 μg/kg (k＝2). When the florfenicol amine residue was 7.25 μg/kg, the expanded uncertainty was 0.94 μg/kg (k＝2). Conclusion The major sources of uncertainty of measurement are the preparation of standards and the fitting of a curve.