中国传统乳制品中乳杆菌的益生菌潜力评估

Evaluation of probiotics potential of Lactobacilli isolated from Chinese traditional dairy products

中文关键词:  乳杆菌  中国传统乳制品  益生菌潜力

英文关键词:Lactobacillus  Chinese traditional dairy products  probiotic potential

中文摘要:

      目的 评估从中国传统乳制品中分离出的9株乳杆菌的功能及其益生菌潜力。方法 从中国传统乳制品中分离纯化得到乳杆菌菌株, 测试其益生菌潜力, 如溶血活性和抗氧化活性等。结果 所有分离的乳杆菌均可耐受低pH值(pH 2.0、2.5和3.0)和高胆盐条件(0.3%、0.5%和1%)。分离的乳杆菌对10种抗生素的敏感性,的测试结果表明, 所有分离株对万古霉素均没有抗性, 此外大多数分离株对青霉素, 氨苄青霉素, 链霉素和四环素具有抗性。所有乳杆菌对HT-29细胞具有优异的粘附能力, 其值介于10.4%~39.0%之间, 并且对DPPH自由基和超氧阴离子自由基具有出色的抗氧化活性。结论 从中国乳制品中分离出的乳杆菌具有作为各种产品中益生菌的潜力。

英文摘要:

      Objective To evaluate the functional and probiotic potential of 9 Lactobacillus strains isolated from Chinese traditional dairy products. Methods The strains were isolated and purified from Chinese traditional dairy products. Probiotic potential of Lactobacillus like hemolytic activity, antioxidant activity and so on was tested. Results All the isolated Lactobacillus strains tolerated low pH values (pH 2.0, 2.5 and 3.0) and high bile salt conditions (0.3%, 0.5% and 1%). The susceptibility of isolated Lactobacillus strains was tested to 10 antibiotics; but the results shows that none of isolates were resistant to vancomycin, while on the other hand most of the isolates were resistant to penicillin, ampicillin, streptomycin, and tetracycline. All Lactobacillus isolates exhibited excellent adherence ability to HT-29 cell with values ranged from 10.4%–39.0% as well as excellent antioxidant activity against DPPH free radical and superoxide anions free radical, respectively. Conclusion The Lactobacillus strains isolated from Chinese dairy products have excellent potential for use as probiotics in various products.