超高效液相色谱-串联质谱法测定火锅底料中柑橘红2号的含量

Determination of citrus red No. 2 in hotpot substrate by super performance liquid chromatography-tandem mass spectrometry

中文关键词:  火锅底料  柑橘红2号  液相色谱串联质谱法

英文关键词:hot pot bottom material  citrus red No.2  liquid chromatography tandem mass spectrometry

中文摘要:

      目的 建立的超高效液相色谱-串联质谱法检测火锅底料中柑橘红2号含量的方法。方法 火锅底料样品用乙腈提取, 氨基固相萃取柱净化, 经Acquity UPLC BEH C18色谱柱分离, 以乙腈与0.1%甲酸水溶液为流动相梯度洗脱, 流速为0.3 mL/min, 多反应监测模式测定, 基质外标法定量。结果 柑橘红2号在1.00～ 40 ng/mL范围内线性关系良好(r＞0.9955), 检出限为4.0 μg/kg。在添加水平分别为8.0、25、100 μg/kg时, 加标回收率为82.2%～86.4%, 相对标准偏差为1.17%～3.20%。结论 该方法准确可靠、灵敏度高, 适用于火锅底料中柑橘红2号的检测。

英文摘要:

      Objective To establish a method for the determination of citrus red No.2 in hot pot bottoms by ultra-performance liquid chromatography-tandem mass spectrometry. Methods Hot pot samples were extracted with acetonitrile, and purified by amino solid phase extraction column, and separated by Acquity UPLC BEH C18 chromatographic column using acetonitrile and 0.1% formic acid aqueous solution as mobile phase for gradient elution, the flow rate was 0.3 mL/min. The samples were detected by multi-reaction monitoring mode, quantified matrix external standard method. Results Citrus red No.2 had a good linear relationship in the range of 1.00? 40 ng/mL (r>0.9955), and the limit of detection was 4.0 μg/kg. When the addition levels were 8.0, 25, and 100 μg/kg, the recovery rates of standard addition were 82.2%?86.4%, and the relative standard deviations were 1.17%?3.20%. Conclusion This method is accurate, reliable, and highly sensitive, and is suitable for the detection of citrus red No.2 in hot pot bottoms.