驴乳抑制胰岛素抵抗形成与氨基酸代谢的相关性

Correlation between the inhibition of donkey milk on insulin resistance and the amino acid metabolism

中文关键词:  驴乳  胰岛素抵抗  氨基酸  代谢组学

英文关键词:donkey milk  insulin resistance  amino acids  metabonomics

中文摘要:

      目的 探讨驴乳(donkey milk, DM)抑制高脂饮食诱导的胰岛素抵抗形成与氨基酸代谢的相关性。 方法 将36只C57BL/6N小鼠随机分为空白对照(Con)组, 高脂饮食(high fat diet, HFD)组和HFD+驴乳组。给予空白对照组小鼠普通饲料及生理盐水灌胃, HFD组给予高脂饲料及生理盐水灌胃, HFD+驴乳组给予高脂饲料, 并且每天按0.1 mL/10 g驴乳灌胃。饮食和驴乳干预8周后, 检测各组小鼠血糖血脂水平和胰岛素敏感性, 采用超高效液相色谱-串联质谱测定小鼠血浆靶标氨基酸含量, 结合SIMCA-P软件对氨基酸数据进行PLS-DA分析, 并分析变化的靶标氨基酸与血糖血脂水平的相关性。结果 高脂饮食诱导8周, 与Con组小鼠相比, HFD组小鼠体重、随机血糖(glucose, GLU)、总胆固醇(total cholesterol, TC)水平、口服葡萄糖耐量(oral glucose tolerance test, OGTT)曲线下面积、胰岛素耐量曲线下面积和空腹血清胰岛素水平显著升高(P＜0.05), 胰岛素敏感指数下降(P＜0.05)。与HFD组相比, HFD+驴乳组体重、GLU、TC和甘油三酯(triglyceride, TG)水平、OGTT曲线下面积显著降低(P＜0.05)。与空白对照组小鼠比较, HFD组小鼠血清中丙氨酸、半胱氨酸、脯氨酸、甲硫氨酸含量升高, 赖氨酸含量降低(P＜0.05); 与HFD组比较, HFD+驴乳组中的半胱氨酸含量显著降低(P＜0.01)。PLS-DA分析结果显示驴乳给予后小鼠血清氨基酸代谢谱较模型组发生了显著变化。Pearson相关分析显示, 脯氨酸、甲硫氨酸和半胱氨酸分别与空腹血糖(fasting blood glucose, FBG)、GLU、TC水平呈显著性正相关(P＜0.05)。结论 驴乳能够延缓高脂饮食诱导小鼠胰岛素抵抗的发生, 并且驴乳能够影响胰岛素抵抗小鼠的血清氨基酸代谢谱, 被改变的半胱氨酸、脯氨酸、甲硫氨酸的代谢与胰岛素抵抗的发生发展密切相关。

英文摘要:

      Objective To investigate the correlation between the inhibition of donkey milk(DM) on insulin resistance induced by high fat diet and amino acid metabolism. Methods 36 C57BL/6N mice were randomly divided into blank control group, high-fat diet (HFD) group and HFD+donkey milk group. The mice in the blank control group were given normal feed and normal saline, the HFD group was given high-fat feed and normal saline, and the HFD+ donkey milk group was given high-fat feed, and 0.1 mL/10 g donkey milk was given by gavage daily. After 8 weeks of dietary and donkey milk intervention, the blood glucose and lipid levels and insulin sensitivity of the mice in each group were detected. UPLC-MS / MS was used to determine the target amino acid content in the plasma of mice, and the amino acid data was combined with SIMCA-P software for PLS-DA analysis. And analyze the correlation between the changed target amino acid and blood glucose and lipid levels. Results The high-fat diet was induced for 8 weeks. Compared with the Con group, the HFD group mice wight, random blood glucose (GLU), total cholesterol (TC) levels, oral glucose tolerance (OGTT) area under the curve, insulin tolerance curve area and fasting serum insulin level were increased significantly (P<0.05), insulin sensitivity index was decreased (P<0.05). Compared with the HFD group, the levels of GLU, TC and triglyceride (TG) and the area under the OGTT curve of the HFD+donkey milk group were significantly reduced (P<0.05). Compared with the blank control group, the levels of alanine, cysteine, proline, and methionine in the serum of mice in the HFD group increased, while the content of lysine decreased (P<0.05). Compared with the HFD group, the cysteine content in the HFD+donkey milk group was significantly reduced (P<0.01). The results of PLS-DA analysis showed that after donkey milk administration, the serum amino acid metabolic profile of mice changed significantly compared with the model group. Pearson correlation analysis showed that proline, methionine and cysteine were significantly positively correlated with fasting blood glucose (FBG), GLU and TC levels (P<0.05). Conclusion Donkey milk can delay the occurrence of insulin resistance in mice induced by high-fat diet, and donkey milk can affect the serum amino acid metabolism profile of insulin-resistant mice, and the altered metabolism of cysteine, proline and methionine are closely related to the occurrence and development of insulin resistance.