离子色谱法测定海带中甘露醇的含量

Determination of mannitol in Laminaria japonica by ion chromatography

中文关键词:  海带  甘露醇  离子色谱法  脉冲安培检测器

英文关键词:Laminaria japonica  mannitol  ion chromatographic method  pulse ampere detector

中文摘要:

      目的 建立测定海带中甘露醇含量的离子色谱法。方法 通过对色谱柱、流动相等色谱条件优化, 考察对海带中甘露醇及其他组分的分离效果。结果 采用DIONEX CarboPac PA10(4 mm×250 mm)色谱柱, 以H2O:NaOH溶液(c=250 mmol/L) (90:10, V:V)为流动相, 流速为0.80 mL/min, 用脉冲安培检测器检测。在该条件下, 甘露醇在1.01~80.83 μg/mL范围内呈现良好的线性关系, 相关系数为0.9926, 最低检出限为0.03 μg/mL, 最低定量限为0.10 μg/mL, 平均回收率为101.61%。结论 该方法简单、灵敏, 测定结果准确, 是测定海带中甘露醇含量的有效方法。

英文摘要:

      Objective To establish a method for the determination of mannitol in Laminaria japonica by ion chromatographic. Methods The separation effect of mannitol and other components in Laminaria japonica were investigated by optimizing the chromatographic conditions of column and flow equivalence. Results DIONEX CarboPac PA10 (4 mm×250 mm) column was used. H2O:NaOH solution (c=250 mmol/L) (90:10, V:V) was used as the mobile phase, and the flow rate was 0.80 mL/min. Under these conditions, mannitol showed a good linear relationship in the range of 1.01?80.83 g/mL. The correlation coefficient was 0.9926, the minimum limit of detection was 0.03 g/mL, the minimum limit of quantification was 0.10 g/mL, and the average recovery was 101.61%. Conclusion The method is a simple, sensitive, accurate and effective method for detection of mannitol in Laminaria japonica.