超高效液相色谱-紫外/荧光法检测蜂蜜中百里香酚含量

Determination of thymol content in honey by ultra performance liquid chromatography-ultraviolet/fluorescence method

中文关键词:  百里香酚  蜂蜜  超高效液相色谱法  紫外检测法  荧光检测法

英文关键词:thymol  honey  ultra performance liquid chromatography  ultraviolet detection method  fluorescence detection method

中文摘要:

      目的 建立超高效液相色谱-紫外检测法和超高效液相色谱-荧光检测法检测蜂蜜中百里香酚残留量的方法。方法 试样经水溶解定容后, 由0.22 μm滤膜过滤, 经C18色谱柱分离, 以乙腈-水(50:50, V:V)为流动相等度洗脱, 流速0.25 mL/min, 由液相色谱-紫外法和液相色谱-荧光法分别测定, 外标法定量。结果 百里香酚在2.0~40.0 mg/kg的浓度范围内线性关系良好, 紫外法和荧光法的定量限分别为2.0 mg/kg和0.2 mg/kg。在代表性洋槐蜜、油菜蜜、荆条蜜、枣花蜜和椴树蜜中, 紫外法和荧光法添加回收率分别为93.6%～101.6%和97.9%～105.0%, 相对标准偏差分别为0.3%～4.9%和0.2%～2.8%。结论 本方法具有操作简便、快速, 准确度高的优点, 适用于蜂蜜中百里香酚残留量的测定。

英文摘要:

      Objective To establish the methods for determination of thymol residue in honey by ultra performance liquid chromatography-ultraviolet detection and ultra performance liquid chromatography-fluorescence detection. Methods After the sample was dissolved in water to constant volume, it was filtered by 0.22 μm filter membrane, separated by C18 chromatographic column for isocratic elution using acetonitrile-water (50:50, V:V) as the mobile phase, and the flow rate was 0.25 mL/min. The content was determined by liquid chromatography-ultraviolet method and liquid chromatography-fluorescence method, and quantified by external standard method. Results Thymol had a good linear relationship in the concentration range of 2.0?40.0 mg/kg, and the quantification limits of the ultraviolet method and the fluorescence method were 2.0 mg/kg and 0.2 mg/kg, respectively. In the representative acacia honey, rape honey, wattle honey, jujube nectar and linden honey, the recovery rates of ultraviolet and fluorescence methods were 93.6%?101.6% and 97.9%?105.0%, respectively, and the relative standard deviations were 0.3%?4.9% and 0.2%?2.8%. Conclusion This method has the advantages of simple, rapid and high accuracy, and is suitable for the determination of thymol residue in honey.