花生中咪鲜胺及2, 4, 6-三氯苯酚残留量的测定

Determination of prochloraz and 2, 4, 6-trichlorophenol residues in peanut

中文关键词:  气相色谱仪  咪鲜胺  花生  QuEChERS

英文关键词:gas chromatograph  prochloraz  peanut  QuEChERS

中文摘要:

      目的 建立气相色谱法测定咪鲜胺及其代谢产物2,4,6-三氯苯酚在花生中残留的分析方法。方法 样品采用乙腈-乙酸(99:1, V/V)提取, 采用QuEChERS净化方式, 气相色谱仪进行测定。分析了不同溶剂、不同净化方式、不同仪器条件对咪鲜胺及2,4,6-三氯苯酚的提取净化影响。结果 在0.01~1.0 μg/mL线性范围内, 咪鲜胺和2,4,6-三氯苯酚的线性均大于0.999, 检出限为0.008 mg/kg, 在添加水平为0.01、0.1、0.5 mg/kg下, 其回收率为81.2%~93.5%, RSD(n=7)为1.2%~4.9%。结论 该方法具有高效, 快捷, 灵敏, 成本低廉的优点, 适用于花生中咪鲜胺及其代谢产物2,4,6-三氯苯酚的分析。

英文摘要:

      Objective To establish a method for determination of residues of prochloraz and its metabolites 2,4,6-trichlorophenol in peanut by gas chromatographic. Methods The samples were extracted by acetonitrile-acetic acid (99:1 volume ratio), purified by QuEChERS and determined by gas chromatograph. The effects of different solvents, different purification methods and different instrument conditions on the extraction and purification of prochloraz and 2,4, 6-trichlorophenol were analyzed. Results The linear range of prochloraz and 2,4, 6-trichlorophenol were greater than 0.999 in the range of 0.01?1.0 μg/mL, the limit of detection was 0.008 mg/kg, and the recoveries were 81.2%?93.5% and RSD (n=7) were 1.2%?4.9% at the addition levels of 0.01, 0.1, and 0.5 mg/kg. Conclusion This method has the advantages of high efficiency, high speed, high sensitivity and low cost, and is suitable for the analysis of prochloraz and its metabolites 2,4, 6-trichlorophenol in peanut.