高效液相色谱法测定海带浓缩制品中岩藻黄素的含量

Determination of fucoxanthin in kelp extract powder by high performance liquid chromatography

中文关键词:  岩藻黄素  海带浓缩制品  海带  高效液相色谱法

英文关键词:fucoxanthin  kelp extract powder  kelp  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法测定海带浓缩制品中岩藻黄素含量的方法。方法 采用Thermo BDS HYPERSIL C18 色谱柱, 以0.1%的乙酸胺水溶液、甲醇、乙腈为流动相, 流速1.0 mL/min, 柱温30 ℃, 检测波长450 nm, 进样量20 μL, 外标法定量。结果 岩藻黄素浓度在0.999~9.987 μg/mL范围内, 与峰面积呈良好的线性关系(r=0.9999), 平均加样回收率为98.53%, 相对标准偏差(RSD)为1.1%(n=9)。结论 本研究建立的含量测定方法简便、准确, 重复性好, 可用于海带浓缩制品的质量控制。

英文摘要:

      Objective To establish a method for determination of fucoxanthin in Kelp extract powder by high performance liquid chromatography. Methods The analysis was performed on a Thermo BDS HYPERSIL C18 column (4.6 mm × 250 mm, 5 μm), with the mobile phase comprising of 0.1% amine acetate-metha nol-acet onitrile-flowing at 1.0 mL/min and the temperature of 30 ℃, and the detection wavelength was set at 450 nm. Results The linear range of fucoxanthin was 0.999-9.987 μg/mL (r=0.9999). The average recovery of fucoxanthin was 98.53%, and the relative standard deviations for fucoxanthin was 1.1% (n=9). Conclusion The method is simple and accurate, with good repeatability, which can be used for the quality control of kelp extract powder.