气相色谱-串联质谱法测定动植物油中胆固醇的含量

Determination of cholesterol in animal and vegetable oils by gas chromatography-tandem mass spectrometry

中文关键词:  胆固醇  动植物油  气相色谱-串联质谱法  含量

英文关键词:cholesterol  animal and vegetable oils  gas chromatography-tandem mass spectrometry  content

中文摘要:

      目的 建立气相色谱-串联质谱法测定动植物油中胆固醇含量的分析方法。方法 样品经异辛烷超声提取, 经HP-5ms柱(30 m×0.25 mm, 0.25 μm)分离, 采用气相色谱质谱联用仪检测, 外标法定量。结果 样品在胆固醇浓度0.06061~0.6061 mg/mL之间呈现良好的线性关系, 加标回收率为90.0%~110.0%, 相对标准偏差(relative standard deviation, RSD)为0.7%。结论 本方法具有快速、操作简便、环保, 并具有高效分离、高灵敏度、高精密度等的优点, 满足了现代分析方法的要求, 能够适用于动植物油中胆固醇含量的测定。

英文摘要:

      Objective To establish a method for determination of cholesterol in animal and vegetable oils by gas chromatography-tandem mass spectrometry (GC-MS). Methods The samples were extracted by ultrasound with isooctane, separated by HP-5ms column (30 m×0.25 mm, 0.25 μm), detected by GC-MS, and quantified by external standard method. Results The samples had good linear relationship between cholesterol concentration from 0.0606061 to 0.6061 mg/mL. The average recoveries were 90.0%-110.0%, and the relative standard deviation (RSD) was 0.7%. Conclusion This method has the advantages of rapid, simple operation, environmental protection, high efficiency separation, high sensitivity, high precision, etc., meets the requirements of modern analytical methods, and can be applied to the determination of cholesterol content in animal and vegetable oil.