食品中大肠菌群计数实验室内部比对研究

Coliform colony test and results analysis in food of laboratory internal comparison

中文关键词:  食品  大肠菌群  大肠菌群测试片  内部比对

英文关键词:foodstuff  coliform  3M coliform count plate  internal alignment

中文摘要:

      目的 监控实验室检测质量和校准结果, 对发现的问题及时采取措施, 以保证实验室的良好检测水平。方法 通过采用GB 4789.3-2016《食品安全国家标准 食品微生物学检验 大肠菌群计数》、SN/T 4547-2017《商品化试剂盒检测方法 大肠菌群和大肠杆菌 方法一》2种检测方法, 对同一质控样品进行检测, 对2名实验人员的实验结果进行分析和能力评定。结果 2名检验人员检验结果具有再现性, 2种方法的结果也具有一致性。结论 组织比对活动, 不断对实验中出现的问题进行改进和总结, 积累经验, 可以稳固和进一步提升个人与实验室的能力。2种检测方法均可对于检测大肠菌群的检测, 在实验中体现了大肠菌群测试片法在操作上和时间上有较大优势。

英文摘要:

      Objective To monitor the test quality and calibration results of the laboratory, take timely measures for the problems found to ensure that the laboratory maintains a good test level. Methods Through the use of GB 4789.3-2016 National food safety standards-Food microbiological test coliform count and SN/T 4547-2017 Commercial kit method-Coliforms and escherichia coli-Test method I, the same quality control sample was tested, and the experimental results of two laboratory personnel ability to analyze and assess. Results The results of the two inspectors were reproducible and the results of the two methods are consistent. Conclusion By organizing the comparison activities, constantly improve and summarize the problems in the experiment, and accumulate experience, the ability of the individual and the laboratory can be stabilized and further improved. Both of the two detection methods can detect coliform group, which is reflected in the experiment, but coliform group test tablet method has a great advantage in operation and time.