不同增菌液对单增李斯特菌的增菌效果比较

Comparison of the effects of different enrichment mediums on the growth of Listeria monocytogenes

中文关键词:  单增李斯特菌  增菌  干扰菌  显色培养基

英文关键词:Listeria monocytogenes  enriched culture  interfering strains  chromogenic medium

中文摘要:

      目的 比较不同选择性增菌液对单增李斯特菌增菌效果及对干扰菌的抗干扰性。方法 参考国标GB/T 4789.30-2016和国际ISO 11290-1-2017, 将2种来源的单增李斯特菌(标准菌株、分离菌株)和2种干扰菌(大肠埃希氏菌、金黄色葡萄球菌)分别接种到4种不同增菌液中, 观察增菌后菌液的生长情况。结果 研究发现低浓度菌液(102数量级), Fraser肉汤对单增李斯特菌的增菌效果及抗干扰性优于LB肉汤。选择性添加剂浓度过高会抑制李斯特菌的生长, 通过选择合适的选择性添加剂及改变添加剂的浓度可以提高检出率。结论 在实验室日常检验过程中, 需考虑样品类别, 选择合适的培养基。提高检测的准确率。

英文摘要:

      Objective To compare the effects of different selective enrichment mediums on the growth of Listeria monocytogenes and the anti-interference ability against interfering strains. Methods According to GB/T 4789.30-2016 and ISO 11290-1-2017, two kinds of Listeria monocytogenes (standard strain, isolated strain) and two kinds of interference bacteria (Escherichia coli, Staphylococcus aureus) were cultured by four different bacterial growth solution to observe the growth of the bacteria. Results When the low-concentration enrichment medium reaches 102 CFU/mL, the study found that Fraser was better than LB broth in increasing bacteria and anti-interference. Too high concentration of selective additives would inhibit the growth of Listeria, and the detection rate could be improved by selecting appropriate selective additives and changing the concentration of additives. Conclusion It is necessary to consider the type of sample and select the appropriate medium to improve the accuracy of detection in the laboratory's daily detection process.