食品中沙门氏菌快速检测技术方法对比结果分析

Comparison of rapid detection techniques of Salmonella in food

中文关键词:  沙门氏菌  VITEK 2 COMPACT  基质辅助激光解吸电离飞行时间质谱法  实时荧光PCR法  环介导等温扩增法  效率

英文关键词:Salmonella  VITEK 2 COMPACT  matrix assisted laser desorption ionization time of flight mass spectrometry  real-time PCR  loop-mediated isothermal amplification  efficiency

中文摘要:

      目的 比较VITEK 2 COMPACT、基质辅助激光解吸电离飞行时间质谱法(matrix assisted laser desorption ionization time of flight mass spectrometry, MALDI-TOF-MS)、实时荧光PCR法(real-time PCR)、环介导等温扩增法(loop-mediated isothermal amplification, LAMP)4类方法, 分析其对沙门氏菌的快速检测效果。方法 挑取12株阳性野生菌株复苏, 配以1株标准菌株作为对照, 分别用VIKEK 2 COMPACT、MALDI-TOF-MS、Real-time PCR、LAMP四类方法对其进行检测, 并比较4类方法的结果差异。结果 Real-time PCR与LAMP单对通用引物能鉴定到属水平, 此外, LAMP测定沙门氏菌时会有假阴性的情况出现; VITEK 2生化鉴定能将少数沙门菌株鉴定到种的水平; 质谱方法能鉴定到亚种水平, 同时会出现鉴定不准确的情况。结论 基于沙门氏菌属水平上, 4类方法都能快速准确鉴定沙门氏菌, 但在使用快速检测技术时均应考量该方法的优点对实际工作的帮助以及缺点对检验结果的影响, 才能提高检测效率。

英文摘要:

      Objective To compare the determination of Salmonella in food by VITEK 2 COMPACT, matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS), real-time PCR and loop-mediated isothermal amplification (LAMP), and analyze their rapid detection effect on Salmonella. Methods 12 positive wild strains of Salmonella were selected for resuscitation with one standard strain of Salmonella as control. They were detected by the methods of VIKEK 2 COMPACT, MALDI-TOF-MS, Real-time PCR and LAMP, and the results of the four methods were compared. Results Real-time PCR and LAMP with single pair of universal primers were used to identify Salmonella at the genus level, and false negative results were observed with LAMP; VITEK 2 COMPACT identified a few Salmonellas at the species level; Salmonella could be identified on subspecies level with MALDI-TOF-MS, and there will be inaccurate identification. Conclusion Based on Salmonella at the genus level, the four kinds of methods can identify Salmonella quickly and accurately, but the advantages of the method to the actual work and the impact of the shortcomings on the test results should be considered when using the rapid detection technology, that can improve the detection efficiency.