复配药材对眼部光氧化应激的预防作用

Preventive effect of compound herbal medicine on photo-oxidative stress of eyes

中文关键词:  熟地黄  枸杞子  菊花  铁皮石斛  复配  抗氧化

英文关键词:Prepared rehmannia root  Fructus lycii  Chrysanthemum  Dendrobium candidum  compound  antioxidant

中文摘要:

      目的 探讨各复配组方对眼睛的保护作用。方法 以光损伤小鼠为模型, 分别用熟地黄+枸杞子、熟地黄+菊花、熟地黄+铁皮石斛和熟地黄+枸杞子+菊花4种复配粗提物对小鼠进行干预, 连续8周后强光造模, 进行视网膜电图(electroretinogram, ERG)检测并采集视网膜进行抗氧化检测。结果 ERG结果显示, 与模型组比较, 药物干预组b波振幅均升高(P＜0.05)。与两两复配组比较, 熟地黄+枸杞子+菊花组b波振幅高于两两复配组。视网膜抗氧化结果显示, 与模型组比较, 复配药物干预均可降低小鼠视网膜丙二醛(malondialdehyde, MDA)含量(P＜0.05), 升高超氧化物歧化酶(superoxide dismutase, SOD)、过氧化氢酶(catalase, CAT)和谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-Px)活力(P＜0.05), 且熟地黄+枸杞子+菊花组的抗氧化效果优于两两复配组。结论 与模型组比较, 各复配物均能够提高ERG的b波振幅和视网膜抗氧化能力, 复配粗提物可能对氧化应激诱导的视疲劳等眼部疾病的发生具有缓解作用。

英文摘要:

      Objective To explore the protective effect of compound prescription on eyes. Methods Photodamaged mice were used as models. Four kinds of crude extracts of Prepared rehmannia root + Fructus lycii, Prepared rehmannia root + Chrysanthemum, Prepared rehmannia root + Dendrobium candidum, and Prepared Rehmannia root + Fructus lycii + Chrysanthemum were employed to interfere with the model of light injury in mice. After 8 weeks, the model was designed by strong light. The electroretinogram (ERG) was detected and the retina was collected for antioxidant detection. Results The results of ERG showed that the amplitude of b-wave in the drug intervention group was higher than that in the model group (P<0.05). Compared with the pairwise group, the amplitude of b wave in the Prepared rehmannia root + Fructus lycii + Chrysanthemum group was higher than that in the pairwise group. The results of retinal antioxidation indicated that compared with the model group, compound drugs could decrease the content of malondialdehyde (MDA) and increase the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in retina of mice, and the antioxidant effect of Prepared rehmannia root + Fructus lycii + Chrysanthemum group was better than that of compound group. Conclusion Compared with the model group, all the compounds can improve the b-wave amplitude of ERG and the antioxidant capacity of retina, and the compound crude extract may alleviate the occurrence of ocular diseases, such as visual fatigue induced by oxidative stress.