响应面法优化柠檬苦素降解酶的固定工艺

Optimization of the immobilization process of limonin degrading enzyme by response surface methodology

中文关键词:  柠檬苦素降解酶  固定化  聚乙烯醇  海藻酸钠  响应面法

英文关键词:limonin degradation enzyme  immobilization  polyvinyl alcohol  sodium alginate  response surface methodology

中文摘要:

      目的 采用响应面法优化柠檬苦素降解酶的固定工艺。方法 采用海藻酸钠-聚乙烯醇固定柠檬苦素降解酶, 通过单因素和响应面实验对固定化条件进行优化, 确定最佳的工艺参数。结果 优化后的工艺参数为: 以聚乙烯醇(0.2 g/100 mL)-海藻酸钠(5.0 g/100 mL)为载体, 戊二醛(2 mL/100 mL)为交联剂, 采用包埋法固定柠檬苦素降解酶, 其中粗酶液浓度0.06 mg/mL、CaCl2浓度6 mg/mL、固定化时间22 h。此条件下的柠檬苦素降解率为(97.87±0.32)%。结论 经优化后的工艺生产出的酶降解率高并且易于分离, 具有很好的实际应用价值。

英文摘要:

      Objective To optimize the immobilization process of limonin degrading enzyme by response surface methodology. Methods Limonin degradation enzyme was fixed with sodium alginate and polyvinyl alcohol, and single factor and response surface experiments were used to optimize the immobilization conditions for determining the best process parameters. Results The optimized process parameters were as follow: 0.2 g/100 mL polyvinyl alcohol and 5.0 g/100 mL sodium alginate were used as carriers, and 2 mL/100 mL glutaraldehyde was used as cross-linking agent, and immobilization of limonin degrading enzyme was based on embedding method. The concentration of crude enzyme solution was 0.06 mg/mL, the concentration of CaCl2 was 6 mg/mL, and the immobilization time was 22 h. The predicted degradation rate of lemon bitters under this condition was (97.87±0.32)%. Conclusion The optimized enzyme produced by the optimized process has high degradation rate and is easy to separate, which has good practical application value.