| 王素钦,陶思依,宋立荣,甘南琴.高效液相色谱-二极管阵列检测法和超高效液相色谱-串联质谱法检测鱼腥藻毒素的比较研究[J].食品安全质量检测学报,2020,11(12):3982-3991 |
| 高效液相色谱-二极管阵列检测法和超高效液相色谱-串联质谱法检测鱼腥藻毒素的比较研究 |
| Comparation study on the determination of anatoxins by high performance liquid chromatography-photodiode array detector and ultra performance liquid chromatography-tandem mass spectrometry |
| 投稿时间：2020-05-20 修订日期：2020-06-12 |
| DOI： |
| 中文关键词: 鱼腥藻毒素 高效液相色谱-二极管阵列检测法 超高效液相色谱-串联质谱法 |
| 英文关键词:anatoxins high performance liquid chromatography- photodiode array detector ultra performance liquid chromatography tandem mass spectrometry |
| 基金项目:中国科学院水生生物研究所特色所项目(Y85Z061601)、湖南文理学院博士启动基金(18BSQD18) |
|
|
| Author | Institution |
| WANG Su-Qin | State Key Laboratory of Fresh Water Ecology and Biotechnology,Institute of Hydrobiology, Chinese Academy of Sciences；Hunan Provincial Key Laboratory for Health Aquaculture and Product Processing in Dongting Lake Area, Hunan Provincial Key Laboratory for Molecular Immunity Technology of Aquatic Animal Diseases, Hunan University of Arts and Science |
| TAO Si-Yi | State Key Laboratory of Fresh Water Ecology and Biotechnology,Institute of Hydrobiology, Chinese Academy of Sciences |
| SONG Li-Rong | State Key Laboratory of Fresh Water Ecology and Biotechnology,Institute of Hydrobiology, Chinese Academy of Sciences |
| GAN Nan-Qin | State Key Laboratory of Fresh Water Ecology and Biotechnology,Institute of Hydrobiology, Chinese Academy of Sciences |
|
| 摘要点击次数: 711 |
| 全文下载次数: 625 |
| 中文摘要: |
| 目的 比较高效液相色谱-二极管阵列检测法(high performance liquid chromatography-photodiode array detector, HPLC-PDA)和超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry, UPLC-MS/MS)对鱼腥藻毒素的检测。方法 样品经酸性甲醇提取后使用超滤管去除杂质, 采用HPLC-PDA和UPLC-MS/MS检测样品中鱼腥藻毒素AnTX-a及HAnTX-a的含量。结果 2种方法均能有效分离分析AnTX-a及HAnTX-a, 且均能排除样品中L-苯丙氨酸的干扰。HPLC-PDA检测法对AnTX-a及HAnTX-a的检测限分别为0.052和0.070 μg/mL; UPLC-MS/MS检测法对AnTX-a及HAnTX-a的检测限均为0.010 ng/mL。结论 2种方法准确、灵敏度高, UPLC-MS/MS较HPLC-PDA检测法具有高灵敏度、高检测效率的优势, 但HPLC-PDA检测法更具普遍适用性。 |
| 英文摘要: |
| Objective To compare the determination of anatoxins by high performance liquid chromatography-photodiode array detector (HPLC-PDA) and ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Methods Samples were extracted by acidified methanol, then purified by ultrafiltration tube to remove the impurities, and the content of AnTX-a and HAnTX-a was detected by HPLC-PDA and UPLC-MS/MS, respectively. Results Both of the 2 methods could effectively separate and analyze AnTX-a and HAnTX-a, and could eliminate the interference of L-phenylalanine in the sample. The detection limit of HPLC-PDA for AnTX-a and HAnTX-a were 0.052 and 0.070 μg/mL, while the detection limit of UPLC-MS/MS for both AnTX-a and HAnTX-a were 0.010 ng/mL. Conclusion The 2 methods are accurate and sensitive. UPLC-MS/MS has the advantages of high sensitivity and high detection efficiency compared with HPLC-PDA, but HPLC-PDA is more universal. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
