光石韦中芒果苷和异芒果苷的一测多评含量测定方法的建立

Establishment of a method for the determination of mangiferin and ismangiferin in Pyrrosiacalvata (Bak.) Ching by quantitative analysis of multi-components by single marker

中文关键词:  光石韦  芒果苷  异芒果苷  一测多评

英文关键词:pyrrosiacalvata  mangiferin  isomangiferin  quantitative analysis of multi-components by single marker

中文摘要:

      目的 建立光石韦中芒果苷和异芒果苷的一测多评含量测定方法。方法 采用高效液相色谱法(high performance liquid chromatography, HPLC), 十八烷基硅烷键合硅胶为固定相的色谱柱(4.6 mm×250 mm, 5 μm), 乙腈-0.1%甲酸(12:88, V:V)为流动相; 检测波长为256 nm。流速1.0 mL/min; 柱温30 ℃, 相对校正因子0.894, 相对保留时间1.18 s。结果 对9批光石韦药材含量测定, 分别用外标法和一测多评法计算芒果苷和异芒果苷含量, 结果两种方法计算结果差异极小。结论 该一测多评法准确、简便可用于光石韦中芒果苷和异芒果苷的含量测定。

英文摘要:

      Objective To establish a method for the determination of mangiferin and ismangiferin in Pyrrosiacalvata (Bak.) Ching. by quantitative analysis of multi-components by single marker. Methods High performance liquid chromatography (HPLC) was used with acetonitrile-0.1% formic acid (12:88, V:V) as mobile phase in a column (4.6 mm×250 mm, 5 μm) and octylsilane bonded silica gel as stationary phase. The detection wavelength was 256 nm. The flow rate was 1.0 mL/min. The column temperature was 30 ℃, the relative correction factor was 0.894, and the relative retention time was 1.18 s. Results For the determination of the contents of 9 batches of Pyrrosiacalvata (Bak.) Ching, the contents of mangiferin and ismangiferin were calculated by using external standard method and quantitative analysis of multi-components by single marker method respectively. Conclusion The method is accurate and simple for the determination of mangiferin and ismangiferin in Pyrrosiacalvata (Bak.) Ching.