超声提取-离子色谱-脉冲积分安培检测法检测黑果腺肋花楸果中的8种单糖和双糖

Determination of 8 kinds of monosaccharides and disaccharides in Aornial mealnocarpa fruit by ultrasonic extraction-ion chromatography-pulse integral ampere detection

中文关键词:  离子色谱法  黑果腺肋花楸果  单糖  双糖

英文关键词:ion chromatography  Aornial mealnocarpa fruit  monosaccharides  disaccharides

中文摘要:

      目的 建立超声提取-离子色谱-脉冲积分安培检测法测定黑果腺肋花楸果中的8种单糖和双糖的方法。方法 采用水浸提超声提取及膜过滤法前处理黑果腺肋花楸果样品, 以Dionex CarboPac PA10 Analytical Column(4 mm×250 mm)阴离子交换色谱柱为分析柱, 以Dionex CarboPac PA10 Guard Column(4 mm×50 mm)为保护柱, 以NaOH与CH3COONa的组合溶液作为流动相, 采用梯度淋洗分离程序, 脉冲积分安培方法检测。结果 8种单糖和双糖的检出限分别为5、8、3、4、7、2、5、2 μg/L, 且均具有较宽的线性范围(0.05~20 mg/L)。黑果腺肋花楸果中单糖和双糖测定的相对标准偏差在0.96%~6.12%之间, 8种单糖和双糖的加标回收率达到90.5%~102.1%。结论 本方法同时检测黑果腺肋花楸果中的单糖和双糖, 其方法简便快捷、分离效果好、基体干扰少、无需衍生、灵敏度高, 适用于黑果腺肋花楸果中的常见单糖和双糖组分的分析。

英文摘要:

      Objective To establish a method for the determination of eight kinds of monosaccharides and disaccharides in Aornial mealnocarpa by ultrasonic extraction, ion chromatography-pulse-integral amperometric detection. Methods The samples of Aornial mealnocarpa fruit were pretreated by ultrasonic extraction with water extraction and membrane filtration. The analytical column was Dionex CarboPac PA10 Analytical Column (4 mm×250 mm), the column Dionex CarboPac PA10 Guard Column(4 mm×50 mm) was used as the protective column. The mobile phase was NaOH and CH3COONa, gradient elution procedure was used for separation and pulse integral amperometric for detection. Results The detection limits of 8 kinds of monosaccharides and disaccharides were 5, 8, 3, 4, 7, 2, 5, and 2 μg/L, respectively, and all of them had a wide linear range of 0.05-20 mg/L. The relative standard deviation of disaccharide determination was 0.96%-6.12%, and the recoveries of 8 kinds of monosaccharides and disaccharides were 90.5%-102.1%. Conclusion The method can be used to detect monosaccharide and disaccharide in Aornial mealnocarpa fruit at same time. It is simple, rapid, effective, less matrix interference, derivative-free and highly sensitive, and is suitable for the analysis of common monosaccharides and disaccharides components in Aornial mealnocarpa fruit.