碳同位素比率法检测分析市售蜂蜜碳-4糖浆的 掺假情况

Detection and analysis of adulteration of carbon-4 syrup in honey by carbon isotope ratio method

中文关键词:  蜂蜜掺假  同位素质谱法  检测

英文关键词:adulterated honey  isotope mass spectrometry  test

中文摘要:

      目的 优化稳定碳同位素比率法, 分析蜂蜜市场质量状况。方法 蜂蜜用水混匀后, 利用钨酸钠溶液和硫酸溶液沉淀蛋白, 多次洗涤后冷冻干燥获得蜜蛋白, 利用同位素质谱仪同时检测蜜蛋白和蜂蜜δ13C值, 用两点标准漂移校准进行数据处理分析, 测得碳-4植物糖浆含量。结果 对来自19个省的137批样品进行检测, 有15批蜂蜜不符合我国蜂蜜产品行业标准的真实性要求, 检出碳-4植物糖浆掺假情况, 不合格率10.95%。结论 检测方法优化后, 检测时间缩短, 蛋白提取成功率提高。通过对实际样品的分析, 发现目前市售蜂蜜质量不容乐观。

英文摘要:

      Objective To optimize the stable carbon isotope ratio method, and analyze the quality of honey market. Methods After the honey was mixed with water, the protein was precipitated by sodium tungstate solution and sulfuric acid solution. After repeated washing, the protein was obtained by freeze drying. Isotope mass spectrometer was used to detect the δ13C value of honey protein and honey simultaneously. Data processing and analysis were performed by two-point standard drift calibration, and the content of C-4 plant syrup was measured. Results Totally 137 batches of samples from 19 provinces were tested, 15 batches of honey did not meet the authenticity requirement of Chinese honey product industry standard, and the adulteration of carbon-4 plant syrup was detected, the unqualified rate was 10.95%. Conclusion The optimized detection method can shorten the detection time and improve the success rate of protein extraction. Through the analysis of actual samples, it is found that the quality of honey on the market is not optimistic.