2018年6~10月河北省市售猪肉馅及冷鲜鸡中沙门氏菌污染情况调查与分析

Investigation and analysis of Salmonella contamination in pork stuffing and whole chicken sold in Hebei province from June to October in 2018

中文关键词:  河北省  猪肉馅  冷鲜鸡  沙门氏菌

英文关键词:Hebei province  pork stuffing  cold chicken  Salmonella

中文摘要:

      目的 了解河北省市售猪肉馅及冷鲜鸡中沙门氏菌污染情况, 掌握部分河北省沙门氏菌阳性分布情况, 为日后监管部门防控沙门氏菌提供参考依据。方法 2018年6~10月从河北省部分地区抽取市售猪肉馅及冷鲜鸡进行调查, 称取25 g左右的猪肉馅置于225 mL的缓冲蛋白胨水, 同时使用缓冲蛋白胨水反复冲洗冷鲜鸡。分别取0.1 mL前菌液置于氯化镁孔雀绿肉汤中培养[(42±0.5) ℃, 24 h], 移除增菌液, 并用木糖赖氨酸脱氧胆盐和亚硫酸铋进行划线分离, 使用VITEK全自动微生物分析系统对可疑菌落进行鉴定。结果 共检测出沙门氏菌阳性样品144份, 阳性检出率为63%, 其中猪肉馅阳性75份, 检出率为65%, 冷鲜鸡阳性数69份, 检出率为60%。猪肉馅和冷鲜鸡6~8月的阳性检出率均高于9~10月份。结论 河北省猪肉馅及整鸡中沙门氏菌阳性的检出率较高, 并且随温度的变化而改变, 应对鸡肉、猪肉的来源、运输和售卖场所处环境进行监督, 以降低沙门氏菌的污染机率。

英文摘要:

      Objective To understand the situation of the Salmonella contamination in pork stuffing and cold chickens sold in parts of Hebei province, and grasp the positive distribution of Salmonella in some Hebei province, so as to provide reference for future regulatory authorities to prevent and control Salmonella. Methods From June to October 2018, the market pork stuffing and cold fresh chicken were selected from some areas of Hebei province for investigation. Approximately 25 g pork stuffing was weighted and put in 225 mL buffered peptone water, and at the same time, the buffered peptone water was used to rinse the chilled chicken repeatedly. 0.1 mL former bacterial solution was taken and cultured in magnesium chloride malachite green broth [(42±0.5) ℃, 24 h]. The enrichment solution was removed and xylose lysine deoxycholate and bismuth sulfitet were used for scribing. VITEK automatic microbiological analysis system was used to identify suspicious colonies. Results A total of 144 Salmonella positive samples were detected, with a positive detection rate of 63%. There were 75 positive samples of pork stuffing, the positive detection rate was 65%, and 69 positive samples of Salmonella in cold chickens, the positive detection rate was 60%. The positive rates of pork stuffing and chilled chicken from June to August were higher than those from September to October. Conclusion The detection rate of Salmonella in pork stuffing and cold chickens in Hebei province is high, and it changes with the temperature. The source, transportation and sale place of chicken and pork should be supervised to reduce the probability of Salmonella pollution.