高效液相色谱-荧光检测法检测植物性食品中14种欧盟优控多环芳烃

Determination of 14 European Union superior controlled polycyclic aromatic hydrocarbons in plant-orgin foods by high performance liquid chromatography- fluorescence detector

中文关键词:  多环芳烃  植物性食品  欧盟  高效液相色谱-荧光检测法

英文关键词:polycyclic aromatic hydrocarbons  plant-orgin foods  European Union  high performance liquid chromatography-fluorescence detector

中文摘要:

      目的 建立高效液相色谱-荧光检测法测定植物性食品如蔬菜、水果及粮食中多环芳烃含量, 了解河北省内上述食品中14种欧盟优控多环芳烃的实际污染状况。方法 样品经环己烷-乙酸乙酯(1:1, V:V)溶液超声提取后, 浓缩至近干, 经正己烷复溶后通过多环芳烃专用固相萃取柱进行净化处理, 洗脱液经浓缩后用乙腈定容, 采用高效液相色谱-荧光检测法进行检测。结果 14种化合物的线性范围为1.00~18.0 μg/kg; 方法回收率为60.9%~125.4%, 相对标准偏差为2.2%~7.6%; 苯并(c)芴、苯并(a)蒽、?、5-甲基-1,2-苯并菲、苯并(j)荧蒽、苯并(b)荧蒽、苯并(k)荧蒽、苯并(a)芘、二苯并(a,e)芘、二苯并(a,i)芘、二苯并(a,h)芘检出限为0.15 μg/kg; 二苯并(a,l)芘、二苯并(a, h)蒽、苯并(g,h,i)苝、茚并(1,2,3-cd)芘检出限为0.30 μg/kg。市售的96份样品中, 检出不同种类不同含量的多环芳烃类化合物, 检出率为51.0%。结论 本方法重现性好, 检测灵敏度高, 可用于水果、蔬菜及粮食中14种多环芳烃的检测。

英文摘要:

      Objective To establish a method for the determination of polycyclic aromatic hydrocarbons (PAHs) in plant-orgin foods including vegetables, fruits and cereal products by high performance liquid chromatography-fluorescence detector(HPLC-FLD), and understand the actual pollution status of the 14 EU-controlled polycyclic aromatic hydrocarbons in the above-mentioned foods in Hebei province. Methods PAHs were extracted by ultrasonic extraction with cyclohexane-ethyl acetate(1:1, V:V), concentrated to nearly dry, redissolved with n-hexane and then purified by special purification column for PAHs. The eluent was concentrated, and the volume was fixed with acetonitrile. The contents of 14 PAHs were determined by HPLC-FLD. Results The linear range of 14 compounds was 1.00?18.0 μg/kg. The recovery rate was 60.9%?125.4%, and the relative standard deviation was 2.2%?7.6%. The detection limits of target compounds such as benzo (c) fluorene, benzo (a) anthracene, chrysene, 5-methyl chrysene, benzo (j) fluoranthene, benzo (b) fluoranthene, benzo (k) fluoranthene, benzo (a) pyrene, dibenzo (a,e) pyrene, dibenzo (a,i) pyrene and dibenzo (a,h) pyrene were 0.15 μg/kg. The detection limits of dibenzo (a,l) pyrene, dibenzo (a,h) fluoranthene, benzo (g,h,i) perylene, indene (1,2,3-cd) pyrene were 0.30 μg/kg. A total of 96 samples were collected, and the pollution of PAHs were detected in various foods on the market, and the detection rate was 51.0%. Conclusion The method has good reproducibility and high sensitivity, and can be used for the determination of 14 PAHs in vegetables, fruits and cereal products.