超声提取柿果皮总三萜酸工艺优化

Optimization of ultrasonic extraction technology of total triterpenoid acid from persimmon peel

中文关键词:  柿果皮  总三萜酸  超声提取  正交法

英文关键词:persimmon peel  ultrasonic extraction  total triterpenoid acid  orthogonal design

中文摘要:

      目的 采用正交法优化柿果皮中总三萜酸的超声提取工艺。方法 以“牛心柿”柿果皮为原料, 利用紫外分光光度计法测定柿果皮中总三萜酸的含量, 并从提取溶剂选择及其浓度、料液比、超声时间和温度等方面, 通过单因素实验和正交试验确定超声提取柿果皮总三萜酸的最佳条件。结果 最佳超声提取工艺为: 在超声功率240 W的前提下, 提取溶剂为75%(V/V)的乙醇、料液比1:15(m/V)、时间45 min、温度50 ℃。在此条件下, 柿果皮总三萜得率最高可达39.33 mg/g。结论 优化后的方法简单、快速、准确、提取率高, 适合提取柿果皮的总三萜酸。

英文摘要:

      Objective To optimize the ultrasonic extraction technology of total triterpenoid acid in persimmon peel by orthogonal design. Methods With the peel of “Niuxinshi” as raw material, the total triterpenoid acid in persimmon peel was determined by spectrophotometer, and the best conditions of extracting total triterpenoid acid from persimmon peel was determined by single factor experiment and orthogonal experiment from the aspects of solvent selection and concentration, material liquid ratio, ultrasonic time and temperature. Results The optimal ultrasonic extraction conditions was as follow: under the condition of 240 W ultrasonic power, 75%(V/V)ethanol as the extraction solvent, the solid-liquid ratio 1:15(m/V), the extraction time 45 min and the temperature 50 ℃. Under this condition, the total triterpenoid acid yield in persimmon peel was up to 39.33 mg/g. Conclusion The optimized method is simple, rapid, accurate and suitable for the extraction of total triterpenoid acid from persimmon peel.