顶空-气相色谱-质谱法测定食用植物油中苯残留量

Determination of benzene residual in edible vegetable oils by headspace gas chromatography-mass spectrometry

中文关键词:  食用植物油  苯残留量  顶空气相色谱质谱  内标法

英文关键词:edible vegetable oil  benzene residual  headspace gas chromatography-mass spectrometry  internal standard

中文摘要:

      目的 建立顶空气相色谱-质谱法测定食用植物油中苯残留量的分析方法。方法 样品经顶空处理后, 注入气相色谱-质谱仪进行检测。使用样品空白基质配制标准系列, 并通过使用内标提高定性与定量的准确性。对顶空前处理及色谱及质谱检测参数进行优化。结果 考察了不同的顶空平衡温度、平衡时间, 以80 ℃、30 min作为优化的顶空参数; 验证了不同种类植物油基质、不同添加水平的方法准确性和精密度, 在4~ 200 μg/kg范围内线性关系良好, 定量限可达0.42~1.98 μg/kg, 检出限可达0.13~0.60 μg/kg。结论 本方法快速简便, 定性定量准确, 能够满足食用植物油中苯残留的检测需要。

英文摘要:

      Objective To establish a method for the determination of benzene residual in edible vegetable oil by headspace gas chromatography-mass spectrometry (HS GC-MS). Methods After headspace treatment, the samples were injected into gas chromatography-mass spectrometer for detection. Standard series were prepared using sample blank matrix. The qualitative and quantitative accuracy were improved by using internal standards. The parameters of headspace processing and chromatogram and mass spectrometry were optimized. Results Different headspace equilibrium temperature and time were investigated, and 80 ℃ and 30 min were used as the optimized headspace parameters. The accuracy and precision of the methods for different vegetable oil substrates and different addition levels were verified. The linear relationship was good in the range of 4–200 μg/kg. The limits of quantitative were 0.42–1.98 μg/kg, and limits of detection were 0.13–0.60 μg/kg. Conclusion This method is fast, simple, quantitative and accurate, and can meet the detection needs of benzene residue in edible vegetable oil.