BAX Q7系统快速检测食品中金黄色葡萄球菌效果验证

Validation of BAXQ7 system for rapid detection of Staphylococcus aureus in food

中文关键词:  BAX Q7全自动病原菌检测系统  金黄色葡萄球菌  国家标准方法  快速检测

英文关键词:BAX Q7 automatic pathogen detection system  Staphylococcus aureus  national standard method  rapid detection

中文摘要:

      目的 验证BAX Q7全自动病原菌检测系统快速检测食品中金黄色葡萄球菌的效果, 以强化食品检验机构对金黄色葡萄球菌的快速检测能力和推进快速检测技术的有效应用。方法 通过BAX Q7全自动病原菌检测系统和GB 4789.10-2010《食品安全国家标准-食品微生物学检验-金黄色葡萄球菌检验》2种方法同步检测定量污染的样品, 比较2种方法的检测结果的符合率和灵敏度。结果 采用2种方法对24份样本检测的结果基本一致, 金黄色葡萄球菌检测符合率均为100%, 但BAX Q7实时荧光定量PCR法相比国家标准方法检出时间缩短4~6 h, 具有更高的灵敏度, 而且操作简便快速。结论 BAX Q7实时荧光定量PCR系统能在食品安全突发事件中快速锁定病原菌, 为溯源调查指明方向, 应用于食品中金黄色葡萄球菌的快速检测合理可行。

英文摘要:

      Objective To verify the effect of BAX Q7 automatic pathogen detection system for rapid detection of Staphylococcus aureus in food, in order to strengthen the rapid detection ability of food inspection institutions for S. aureus and promote the effective application of rapid detection technology. Methods The BAX Q7 automatic pathogen detection system and GB 4789.10-2010 National food safety standard-Food microbiology examination-Staphylococcus aureus were used to quantitatively detect the food contaminated by S. aureus simultaneously, and the coincidence rate and sensitivity of the detection results of the two methods were compared. Results The detection results of 24 samples were basically the same using the two methods. The coincidence rate of S. aureus detection was 100%, but the detection time of the BAX Q7 real-time quantitative PCR method was 4-6 h, shorter than that of the national standard method, and BAX Q7 had higher sensitivity, specificity, simple and fast operation. Conclusion BAX Q7 quantitative real-time PCR system can quickly identify pathogens in food safety emergencies and indicate the direction for traceability investigations, and it is reasonable and feasible for rapid detection of S. aureus in food.