三氯杀螨醇胶体金免疫快速检测试纸条研制及在茶叶中的应用

Development of dicofol colloidal gold immune rapid detection test strip and its application in tea

中文关键词:  三氯杀螨醇  茶叶  胶体金免疫层析法  快速检测

英文关键词:dicofol  tea  gold immunochromatography assay  rapid detection

中文摘要:

      目的 建立基于胶体金免疫层析法的三氯杀螨醇快速检测试纸条的研制方法, 并探究其在新鲜茶叶中的应用。方法 以三氯杀螨醇为原料, 采用琥珀酸酐对其衍生化合成半抗原; 活化酯法将半抗原与白蛋白偶联得到人工抗原; 用人工抗原免疫小鼠获得单克隆抗体。用上述原料制备出本研究试纸条。结果 所研制试纸条对茶叶中三氯杀螨醇检测限为0.2 mg/kg, 检测时间8 min。可特异性地检测三氯杀螨醇的残留量, 而对其他与三氯杀螨醇结构或功能相似的农药无交叉反应。结论 本研究提供的试纸条适宜基层实验室的大批量样本检测和田间现场快速检测, 能够实现从源头上控制污染茶叶的流通上市。

英文摘要:

      Objective To establish a rapid test strip for dicofol detection based on colloidal gold immunochromatography, and to explore its application in fresh tea. Methods Dicofol was used as raw material and succinic anhydride was used to derivatize it to synthesize hapten. Activated ester method is used to couple hapten with albumin to obtain artificial antigen. Mice were immunized with artificial antigen to obtain monoclonal antibody. The test strip for this study was prepared from the above raw materials. Results The detection limit of dicofol in tea was 0.2 mg/kg and the detection time was 8 min. The residue of dicofol could be specifically detected, but there was no cross reaction to other pesticides with similar structure or function to dicofol. Conclusion The test strip provided in this study is suitable for large-scale sample detection in grass-roots laboratories and rapid detection in the field, and can control the circulation and listing of contaminated tea from the source.