高效液相色谱法测定牛奶中四环素类兽药残留量的不确定度评定

Uncertainty evaluation for the determination of tetracycline drug residues in milk by high performance liquid chromatography

中文关键词:  四环素类  不确定度  高效液相色谱法  牛奶

英文关键词:tetracycline drug residues  uncertainty  high performance liquid chromatography  milk

中文摘要:

      目的 评定高效液相色谱法测定牛奶中四环素类兽药残留量的不确定度。方法 依据GB/T 21317-2007《动物源性食品中四环素类兽药残留量检测方法 液相色谱-质谱/质谱法与高效液相色谱法》, 建立相关数学模型, 对高效液相色谱法实验过程中的不确定度来源进行分析与评定。结果 当高效液相色谱法测定牛奶中土霉素、四环素、金霉素、强力霉素的残留量分别为0.939、0.825、0.838、0.900 mg/kg时, 扩展不确定度分别为0.078、0.073、0.081、0.078 mg/kg (k=2)。结论 检测结果的不确定度主要受标准溶液配制、工作曲线拟合、样品回收率影响。

英文摘要:

      Objective To evaluate the uncertainty of the determination of tetracycline drug residues in milk by high performance liquid chromatography. Methods According to GB/T 21317-2007 Determination of tetracycline residues in food of animal origin by high performance liquid chromatography-tandem mass spectrometry method and high performance liquid chromatography method, relevant mathematical models were established to analyze and evaluate the sources of uncertainty in the high performance liquid chromatography experiment. Results When the content of oxytetracycline, tetracycline, aureomycin, deoxytetracycline in milk was 0.939, 0.825, 0.838, 0.900 mg/kg by high performance liquid chromatography, its extended relative uncertainty was 0.078, 0.073, 0.081, 0.078 mg/kg (k=2), respectively. Conclusion The uncertainty of detection results is mainly affected by standard solution preparation, working curve fitting and sample recovery rate.