乌饭树果乙醇提取物不同极性分部的体外抗氧化活性研究

Study on the in vitro antioxidant activity of different polarity fractions of ethanol extract obtained from Vaccinium bracteatum Thunb. fruits

中文关键词:  乌饭树果  乙醇提取物  极性分部  抗氧化活性

英文关键词:Vaccinium bracteatum Thunb. fruits  ethanol extract  polar fractions  antioxidant activity

中文摘要:

      目的 筛选乌饭树果乙醇提取物中抗氧化活性最强的极性分部。方法 采用石油醚、乙酸乙酯、氯仿、正丁醇和水等5种不同极性溶剂对乌饭树果乙醇提取物进行进一步梯度萃取, 并分析比较其总抗氧化能力(total antioxidant capacity, TAC)、抗脂质过氧化能力及对羟基自由基OH?、超氧阴离子自由基O2-?的清除效果。结果 乌饭树果乙醇提取物的5个不同极性分部均具有一定的抗氧化活性, 但其活性大小差异极显著(P<0.01), TAC大小依次为: 乙酸乙酯部>氯仿部>正丁醇部>石油醚部>水部。同时, 在OH?、O2-?自由基清除能力和抗脂质过氧化能力上, 乙酸乙酯部均优于氯仿部和正丁醇部, 且呈明显的量效关系。结论 乌饭树果乙醇提取物的乙酸乙酯部具有最强的抗氧化活性, 且对OH?、O2-?的清除能力及抗脂质过氧化能力均强于L-抗坏血酸及其它部位, 可作为天然抗氧化剂的潜在有效来源。

英文摘要:

      Objective To screen the strongest antioxidant activities of polarity fraction of ethanol extract obtained from Vaccinium bracteatum Thunb. fruits. Methods Totally 5 different polarity solvents (petroleum ether, ethyl acetate, chloroform, n-butanol and water) were used to furtherly fractionate ethanol extracts from Vaccinium bracteatum Thunb. fruits. Moreover, the total antioxidant capacity (TAC), anti-lipid peroxidation and scavenging effect of 5 kinds of polarity fractions on hydroxyl radical (OH?) and superoxide radical (O2-?) were studied and compared. Results A total of 5 polarity fractions of ethanol extract obtained from Vaccinium bracteatum Thunb. fruits were showed antioxidant activity while exhibited significant differences (P<0.01), among which the ethyl acetate fraction had the strongest TAC, following by the chloroform, n-butanol fractions and the petroleum ether, the water fraction was the weakest. Furthermore, the ethyl acetate fraction had better scavenging capacities of OH?, O2-? and anti-lipid peroxidation than n-butanol fraction and chloroform fraction, and also showed a significant dose-response relationship. Conclusion The ethyl acetate fraction exhibited the strongest of antioxidant capacity, was superior to L-ascorbic acid and other fractions on the scavenging effects of OH?, O2-? and anti-lipid peroxidation, which could be used as a potential effective source of natural antioxidants.