超高效液相色谱法快速测定饮料中13种常见的食品添加剂

Determination of 13 food additives in beverages by ultra performance liquid chromatography

中文关键词:  超高效液相色谱法  饮料  防腐剂  甜味剂  合成着色剂

英文关键词:ultra performance liquid chromatography  beverage  preservative  sweetener  artificial synthetic colorant

中文摘要:

      目的 建立超高效液相色谱法(ultra performance liquid chromatography, UPLC)测定水果茶饮料中常见的13种防腐剂、甜味剂和着色剂的分析方法。方法 样品经超声加热除去二氧化碳或乙醇后稀释、离心、过滤后直接进样, 采用Agilent ZORBAX Eclipse Plus C18(50 mm×2.1 mm, 1.8 μm)色谱柱分离, 以甲醇-乙酸铵 (0.02 mol/L)为流动相进行梯度洗脱, 柱温28 ℃, 流速0.5 mL/min, 采用二极管阵列检测器切换波长分析, 外标法定量。结果 13种待测成分在6 min内良好分离, 质量浓度在0.05~10 μg/mL范围内线性良好, 相关系数r2均大于0.999; 定量限为0.010~0.025 μg/mL; 平均加标回收率在92.1%~101.0%之间; 重复性和精密度RSD(n=6)在5.0%以内。结论 该方法快速、准确, 适合用于饮料中13种防腐剂、甜味剂、合成着色剂定性、定量测定。

英文摘要:

      Objective To establish a method for the qualitative and quantitative detection of 13 common preservatives, sweeteners and colorants in beverages by ultra performance liquid chromatography. Methods After the samples were removed carbon dioxide or ethanol by ultrasound and heating, they were diluted and filtered. Agilent ZORBAX Eclipse Plus C18(50 mm×2.1 mm, 1.8 μm)column was selected for separation, with column temperature of 28 ℃, and the flow rate was set at 0.5 mL/min. Methanol-0.02 mol/L ammonium acetate aqueous solution was used as mobile phase, and the program was performed with gradient elution. External standard method was used for quantitative analysis. Results The 13 components to be tested were well separated within 6 min, the mass concentration was in the range of 0.05?10 μg/mL, and the correlation coefficient r2 was greater than 0.999. The limits of quantitation were 0.010?0.025 μg/mL. The average standard recoveries were between 92.1% and 101.0%. RSDs of repeatability and precision (n=6) were within 5.0%. Conclusion This method is simple, rapid with accurate results, and can be used for the qualitative and quantitative determination of preservatives, sweeteners and artificial synthetic colorants in commercially available beverages.