外源γ-氨基丁酸对双孢蘑菇高CO2保鲜品质及生理生化性质的影响

Effects of exogenous γ-aminobutyric acid on the fresh-keeping quality and physiological and biochemical properties of Agaricus bisporus with high CO2

中文关键词:  γ-氨基丁酸  双孢蘑菇  高CO2  保鲜

英文关键词:γ-aminobotyric acid  Agaricus bisporus  high CO2  preservation

中文摘要:

      目的 探究外源γ-氨基丁酸(γ-aminobotyric acid, GABA)对双孢蘑菇高CO2保鲜品质和生理生化性质的影响。方法 分别用5 mmol/L GABA和0.1 mmol/L γ-乙烯基-γ-氨基丁酸对双孢蘑菇进行预处理, 以蒸馏水处理为对照, 在21% O2+10% CO2气调、温度4 ℃及RH85条件下进行贮藏, 评定其硬度、颜色、总酚、蛋白质、多酚氧化酶(polyphenol oxidase, PPO)、过氧化物酶(peroxidase, POD)、苯丙氨酸解氨酶(phenylalnine ammonialyase, PAL)、H2O2、抗?O2-活力、过氧化氢酶(catalase, CAT)等指标。结果 外源GABA处理可以保持高CO2保鲜条件下的双孢蘑菇的硬度和颜色, 维持较高的总酚和蛋白质含量, 抑制PPO、POD活性, 并提高PAL活性; 同时, 降低H2O2含量, 提高抗?O2-活力和CAT活性; γ-乙烯基-γ-氨基丁酸处理对双孢蘑菇高CO2保鲜效果无显著影响。结论 外源GABA处理可以通过延缓衰老提高双孢蘑菇高CO2保鲜条件下的贮藏品质, 这为GABA协同高CO2保鲜双孢蘑菇提供了理论依据。

英文摘要:

      Objective To explore the effects of exogenous γ-aminobutyric acid (GABA) on the fresh-keeping quality and physiological and biochemical properties of Agaricus bisporus with high CO2. Methods Agaricus bisporus was pretreated with 5 mmol/L GABA and 0.1 mmol/L γ-vinyl-γ-aminobutyric acid, respectively, and distilled water as control. Agaricus bisporus was stored under 21% O2 + 10% CO2, 4 ℃ and RH85. Hardness, color, total phenol, protein, polyphenol oxidase (PPO) activity, peroxidase (POD) activity, phenylalanine ammonia lyase (PAL) activity, H2O2, anti?O2- activity and catalase (CAT) activity were determined. Results Exogenous GABA treatment under high CO2 preservation conditions could maintain the hardness and color of Agaricus bisporus, maintain high content of total phenol and protein, inhibit the activity of PPO and POD, and improve the activity of PAL. at the same time, reduce the content of H2O2, improve the activity of anti ?O2- and CAT activity. The γ-vinyl-γ-aminobutyric acid treatment had no significant effect on the high CO2 preservation effect of Agaricus bisporus. Conclusion Exogenous GABA treatment can improve the storage quality of Agaricus bisporus under the condition of high CO2 preservation by delaying aging, which provide a theoretical basis for GABA and high CO2 preservation of Agaricus bisporus.