黑米多酚对四氧嘧啶诱导糖尿病小鼠的影响

Effect of black rice polyphenols on alloxan induced diabetes in mice

中文关键词:  黑米多酚  糖尿病  血糖

英文关键词:black rice polyphenols  diabetes  serum glucose

中文摘要:

      目的 研究黑米多酚对四氧嘧啶诱导的糖尿病模型小鼠的降血糖作用。方法 以黑米为原料提取纯化黑米多酚, 在应用四氧嘧啶腹腔注射并成功建立高血糖模型小鼠方法的基础上, 将模型组50只雄性小鼠按每组10只, 随机分为阴性对照组(NM)、阳性对照组(PM)及黑米多酚低、中、高剂量组(B1、B2、B3)。另取10只雄性小鼠作为正常对照组(NC), 连续灌喂4周。结果 与正常组相比, 模型组小鼠饮水、体重、血糖水平均有显著差异(P＜0.05), 且血糖水平达到高血糖标准, 说明造模成功; 黑米多酚可以缓解四氧嘧啶造模糖尿病小鼠的症状, 具体表现为减缓多饮、多尿、体重下降的症状; 一定程度上减缓糖尿病小鼠血糖增高的趋势, 且高剂量的黑米多酚可以显著降低糖尿病小鼠的血糖(P＜0.05), 有效刺激小鼠胰岛素的分泌和小鼠肝糖元的产生, 对保护糖尿病小鼠的肝脏和肾脏具有一定的作用。结论 黑米多酚具有降低四氧嘧啶诱导高血糖模型小鼠血糖的作用, 但其机制有待进一步探究。

英文摘要:

      Objective To study the hypoglycemic effect of black rice polyphenol on alloxan induced diabetic mice. Methods Black rice polyphenols were extracted and purified from black rice. On the basis of the method of intraperitoneal injection of alloxan and the establishment of hyperglycemia model mice, 50 male mice in the model group were randomly divided into negative control group (NM), positive control group (PM) and low, medium and high dose groups of black rice polyphenols (B1, B2, B3). Another 10 male mice were taken as the normal control group (NC) and all the groups were administered once a day for 4 consecutive weeks. Results The results showed that compared with the normal group, there were significant differences in drinking water, body weight and blood glucose level in the model group (P<0.05), and the blood glucose level reached the hyperglycemia standard indicating that the model was successful. Black rice polyphenol could alleviate the symptoms of alloxan induced diabetic mice, which was manifested in slowing down the symptoms of polydipsia, polyuria and weight loss; to some extent, it could slow down the trend of blood glucose increase in diabetic mice. High dose of black rice polyphenol could significantly reduce the blood sugar of diabetic mice (P<0.05), and effectively stimulate the secretion of insulin and the production of glycogen in mice liver, which has a certain effect on protecting the liver and kidney of diabetic mice. Conclusion Black rice polyphenol could reduce the blood glucose of hyperglycemic model mice induced by alloxan, but its mechanism should be further explored.