高效液相色谱-蒸发光散射法分析动物油脂种属特征

Analysis species characteristics of animal oils by high performance liquid chromatography-evaporative light scattering method

中文关键词:  甘油三酯  动物油脂  高效液相-蒸发光散射法

英文关键词:triglyceride  fats  high performance liquid chromatography-evaporative light scattering method

中文摘要:

      目的 分析确定5种动物油脂的甘油三酯种属特征。方法 采集5种常见动物油脂(猪、牛、羊、鸡、鸭), 用丙酮提取后直接进样分析, 采用高效液相色谱?蒸发光散射法(high performance liquid chromatography - evaporative light scattering method, HPLC-ELSD), 色谱柱为C18柱, 流动相为二氯甲烷?乙腈-冰乙酸(30:70:0.2, V/V/V), 流速1.0 mL/min, 柱温35 ℃, 蒸发光散射检测器检测。结果 在本文所优化的动物油脂高效液相色谱-蒸发光散射分析条件下, 10种甘油三酯组分都能够得到有效分离, 5种动物油脂的谱图数据特征性强, 且有自己的特征峰, 同种属动物油脂谱图相似性高。结论 甘油三酯可以作为鉴定这5种动物油脂种属的依据, 为市场打假及清真食品检验提供参考方法。

英文摘要:

      Objective To analyze and determine the triglyceride characteristics of 5 kinds of animal fats and oils. Methods The five kinds of common animal fats (pig, cow, sheep, chicken, duck) were collected, extraceted with acetone and analyzed by direct sample injection. High performance liquid chromatography-evaporative light scattering method (HPLC- ELSD) was used. The chromatographic column was C18 column, the mobile phase was dichloromethane - acetonitrile- lacial acetic acid (30:70:0.2, V/V/V) and the flow rate was 1.0 mL/min with 35 ℃ column temperature, and detection by evaporative light scattering detector. Results Under the conditions of HPLC-ELSD of animal fats optimized in this paper, all the 10 triglyceride components could be separated effectively. Spectral data characteristics was strong about 5 animal fats. which have their own characteristic peak. The fats spectra of the same species are highly similar. Conclusion Triglyceride can be used as the basis for the identification of the 5 kinds of animal fat species, which provides a reference method to checkout anti-counterfeiting halal food inspection in the market.