液相色谱法测定核桃乳中糠氨酸含量

Determination of furosine content in walnut milk by liquid chromatography

中文关键词:  糠氨酸  核桃乳  高效液相色谱

英文关键词:furosine  walnut milk  high performance liquid chromatography

中文摘要:

      目的 建立液相色谱法测定核桃乳中糠氨酸含量的检测方法。方法 样品经酸水解、乙酸铵缓冲溶液定容后, 经反相C18色谱柱分离, 以甲醇/三氟乙酸溶液为流动相, 流速为1.0 mL/min, 梯度洗脱, 在紫外检测器波长为280 nm下检测。结果 糠氨酸浓度在2~50 μg/L的范围内线性关系良好, 相关系数均大于0.999。糠氨酸在2.00、5.00和10.00 mg/100 g蛋白质添加水平的回收率为88.82%~91.95%, 相对标准偏差(relative standard deviation, RSD)小于3.55% (n=6), 方法检出限为1.0 mg/100 g蛋白质。结论 该方法操作过程简单、方法准确、可靠、重现性好、回收率较高, 适合于核桃乳中糠氨酸含量的分析检测。

英文摘要:

      Objective To establish a method for the determination of furosine in walnut milk by liquid chromatography. Methods The sample was hydrolyzed by acid and stabilized by the buffer solution of ammonium acetate, separated by reverse phase C18 column at the flow rate of 1.0 mL/min by gradient elution using methanol/trifluoroacetic acid solution as mobile phase, and detected at a UV detector wavelength of 280 nm. Results The furosine had a good linear relationship in concentration range of 2-50 μg/L, and the correlation coefficient was greater than 0.999. The recoveries of furoate at protein levels of 2.00, 5.00 and 10.00 mg/100 g were 88.82%-91.95%, and relative standard deviation was less than 3.55% (n=6). The detection limit of the method was 1.0 mg/100 g protein. Conclusion The method is simple, accurate and reliable, it has a good reproducibility and high recovery rate, and is suitable for the analysis and detection of the content of furosine acid in walnut milk.