高效液相色谱法测定竹木制食品相关产品中7种防菌防腐剂的残留量

Determination of 7 kinds of antibacterial preservatives residues in bamboo and wooden products related food by high performance liquid chromatography

中文关键词:  竹木制食品相关产品  防菌防腐剂  高效液相色谱法

英文关键词:bamboo and wooden products related food  antibacterial preservatives  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法同时测定竹木制食品相关产品中7种防菌防腐剂。方法 样品经甲醇提取, 选用色谱柱Agilent (ZORBAX SB-C18)色谱柱分离, 在0.02 mol/L乙酸铵(含0.03%乙酸)和甲醇溶液为流动相条件下进行梯度洗脱, 采用二极管阵列检测器, 在220 nm波长下检测, 外标法对7种防菌防腐剂的进行定性定量分析。结果 该方法分析时间适中, 7种组分均可分离完全, 线性良好, 相关系数均大于0.990, 检出限为1.0~12.0 mg/kg, 平均回收率为73.96%~108.29%, 相对标准偏差为0.11%~7.78%(n=3)。结论 该方法操作简单, 精密度高, 重复性好, 满足同时检测竹木制食品相关产品中7种防菌防腐剂的检测要求。

英文摘要:

      Objective To establish a method for the simultaneous determination of 7 antimicrobial preservatives in bamboo and wooden products related food by high performance liquid chromatography. Methods The sample was extracted by methanol and separated on an Agilent (ZORBAX SB-C18) chromatographic column by gradient elution using 0.02 mol/L ammonium acetate (containing 0.03% acetic acid) and methanol solution as mobile phase, and the components were detected by diode array detector at 220 nm wavelength. The qualitative and quantitative analysis of 7 kinds of antiseptic and anti-mould agents were realized by external standard method. Results The analysis time of the method was moderate, the 7 components could be separated completely, the linearity was good, the correlation coefficient was greater than 0.990, and the limits of detection were 1.0-12.0 mg/kg. The average recoveries were 73.96%-108.29%, and the relative standard deviation was 0.11%-7.78% (n=3). Conclusion This method has the advantages of simple operation, high precision and good repeatability, and can meet the requirements for the simultaneous detection of 7 antiseptic agents in bamboo and wood products related food.